血管内皮细胞生长因子对肝癌细胞钙粘附蛋白表达的影响  

Effects of VEGF on the expression of homotypic adhesion and E-cadherin of HepG_2 cells

作　　者：毛华[1] 赵敏芳[1] 宋卫生[1] 

机构地区：[1]南方医科大学珠江医院消化内科,广州510515

出　　处：《肝脏》2007年第1期20-21,60,共3页Chinese Hepatology

摘　　要：目的研究血管内皮细胞生长因子(VEGF)对肝癌HepG2细胞同质性粘附作用和钙粘附蛋白(E-cadherin)的影响。方法采用3H-TdR掺入试验测定VEGF对肝癌HepG2细胞同质性粘附作用,激光扫描共聚焦显微镜检测VEGF对肝癌细胞E-cadherin表达作用。结果1ng/ml、5ng/ml VEGF诱导HepG2细胞60min、90min 3H-TdR掺入实验(dpm/min)分别为1758.67±289.46、1380.03±328.55和3124.3±2262.14、2245.6±273.24,10ng/ml VEGF诱导HepG2细胞60min、90min、120min3H-TdR掺入实验分别为1232.32±201.04、2337.5±333.04、2236.99±237.07,显著低于对照组60、90、120分钟的2184.49±336.03、3560.±255.17和4337.4±377.35。5ng/ml VEGF降低肝癌细胞E-cadherin的表达,荧光强度为757±103,显著低于对照组的352±56。结论VEGF降低肝癌细胞同质性粘附作用可能与其降低肝癌细胞E-cadherin有关。Objective To study the effects of vascular endothelial growth factor （VEGF） on the expression of homotypie adhesion and E-eadhefin of hepatocellular carcinoma cells. Methods We measured the function of homotypie adhesion with ^3 H-TdR incorporation and expression of E-eadherin with Laser Scanning Confoeal Microscope. Results After 60,90 rain, the value （dpm/min） of 3 H-TdR incorporated in HepG2 cells induced by 1 ng/ml and 5 ng/ml VEGF were 1 758.67± 289.46,1 380.03±328.55 and 3 124.3 ± 2 262.14,2 245.6 ± 273.24, 10 ng/ml VEGF were 1 232.32 ± 201.04,2 337.5 ± 333.04 and 2 236.99± 237.07,which is lower than that in eontral group. The expression of E-eadherin in hepatocellular carcinoma cells induced by 5 ng/ml VEGF is 757 ± 103 which is much lower than that in eontral group of 352 ± 56. Conclusion VEGF can decrease homotypie adhesion of hepatoeellular eaneinoma cell HepG2 ,which is connected with fewer expression of E-eadherin in hepatocellular eaneinoma cell HepG2 induced by VEGF.

关 键 词：血管内皮细胞生长因子 肝癌细胞株 钙粘附蛋白 

分 类 号：R735.7[医药卫生—肿瘤]