根尖周炎动物模型中CD14、TLR4的表达  

Expression of lipopolysacharide receptors CD14 and TLR4 in a model of rat molar apical periodontitis

作　　者：张琛[1] 侯本祥[1] 

机构地区：[1]首都医科大学口腔医学院牙体牙髓科,100050

出　　处：《中华口腔医学杂志》2007年第3期148-149,共2页Chinese Journal of Stomatology

基　　金：北京市科技新星计划(H013610290113)

摘　　要：目的研究大鼠根尖周炎炎症组织中脂多糖炎症信号受体 CD14、Toll 样受体4(Toll-like receptor 4,TLR4)的表达特点,探讨根尖周炎中脂多糖的信号转导途径。方法建立大鼠磨牙内毒素根尖周炎模型,采用免疫组化染色观察根尖周炎炎症组织中 CD14、TLR4的表达情况,并计算CD14、TLR4的阳性细胞率。结果正常根尖周组织中未发现 CD14和 TLR4免疫阳性细胞,根尖周炎症组织中 CD14和 TLR4表达阳性,CD14、TLR4阳性细胞率差异无统计学意义(P>0.05)。结论与正常根尖周组织相比,炎症根尖周组织中 CD14和 TLR4的表达显著增强,CD14和 TLR4的表达量差异无统计学意义,提示脂多糖可能通过 CD14、TLR4信号受体在根尖周炎症中发挥作用。Objective To characterize the expression of lipopolysacharide （LPS） receptors-CD14 and TLR4 in rat periradieulur tissue and to investigate the signal transduetion pathway of LPS and its receptors in periapieal periodontitis, Methods After establishment of a model of rat molar LPS-indueed apical periodontitis, CD14 and TLR4 expression in healthy and inflammatory periapieal tissue were determined by immunohistochemistry. Results CD14 and TLR4 were not detected in healthy periapial tissue but strongly positive in inflammatory periapieal tissue, Positive cells were mostly monocytes/maerophages. Conclusions CD14 and TLR4 may play an important role in the pathogenesis of periapieal peridontitis, probably via immune cells such as monocytes/maerophages and neutrophils,

关 键 词：根尖周疾病 脂多糖类 抗原 CD14 

分 类 号：R781.341[医药卫生—口腔医学]