靶向转基因抑制涎腺腺样囊性癌增殖的实验研究  

Inhibition of proliferation of salivary adenoid cystic carcinoma by targeting gene transfer

作　　者：苏涛[1] 孙宏晨[1] 臧光祥[1] 张红[2] 卢东民[2] 

机构地区：[1]吉林大学口腔医学院病理科,长春130041 [2]浙江湖州师范学院口腔系

出　　处：《中华口腔医学杂志》2007年第3期184-185,共2页Chinese Journal of Stomatology

基　　金：国家自然科学基金(30672338);教育部高等学校博士学科点专项科研基金(20030183068)

摘　　要：目的探讨人端粒酶逆转录酶(human telomerase reverse transcriptase,hTERT)启动子调控肿瘤坏死因子相关凋亡诱导配体(tumor necrosis factor-related apoptosis-inducing ligand,TRAIL)基因在涎腺腺样囊性癌细胞(salivary adenoid cystic carcinoma,SACC)-83中表达及对 SACC-83增殖的影响。方法脂质体介导 pACTERT-TRAIL 转染 SACC-83和人胚肺成纤维细胞(human embryonic lungfibroblast,HEL),通过 RT-PCR 检测 TRAIL 表达,甲基噻唑基四唑法检测细胞存活率,流式细胞仪检测细胞凋亡率。结果 hTERT 启动子调控 TRAIL 基因在 SACC-83中明显表达,抑制其增殖(存活率62.80%),且诱导细胞凋亡比例增加(10.89%);而在 HEL 细胞中未能诱导 TRAIL 基因表达,对该细胞的增殖和凋亡无明显影响。结论 hTERT 启动子可诱导 TRAIL 基因在 SACC-83中特异性表达及诱导细胞凋亡。Objeclive To investigate the expression of gene TRAIL driven by human telomerase reverse transcriptase （hTERT） promoter in SACC-83 cell tumor necrosis factor related apoptosis in dncing hgand. Methods After pACTERT-TRAIL plasmid transfected was into SACC-83 and HEL cells through liposome, the expression of TRAIL was examined using RT-PCR technique, the cells＇survival rate by methyl thiazolyl tetrazohum（MTT） method and apoptosis rate by flow cytometry. Results Expression of extrinsic TRAIL gene driven by hTERT promoter was detected in SACC-83 cells , and not detected in human embryonic lung fibroblast（HEL） cells. After transfection of pACTERT-TRAIL, the proliferation of SACC-83 cells was significantly inhibited , and its apoptotic rate was promoted, whereas no inhibited effect was observed on HEL cells and its apoptotic rate showed little change. Conclusions hTERT promoter can be used to induce tumor-specific expression of TRAIL gene and apoptosis in SACC-83 cells.

关 键 词：癌 腺样囊性 转基因 端粒酶 

分 类 号：R739.8[医药卫生—肿瘤]