甲胺磷农药降解菌的筛选鉴定及其降解效能研究  

Isolation and identification methamidophos-degrading bacteria and determined the effect of degradation

作　　者：郑永良[1] 刘德立[2] 刘世旺[1] 邹煜平[2] 

机构地区：[1]黄冈师范学院生命科学与工程学院,湖北黄冈438000 [2]华中师范大学生命科学学院,武汉430079

出　　处：《华中师范大学学报（自然科学版）》2007年第1期95-98,共4页Journal of Central China Normal University：Natural Sciences

基　　金：国家自然科学基金资助项目(30170011);武汉市科技攻关项目(20062001018)

摘　　要：分别从黄冈棉田、荆州农田、沙市农药厂等长期受有机磷农药污染的不同环境土壤取样,通过富集培养,筛选分离到10株甲胺磷(MAP)降解菌.测定了各菌株的降解效能.选取降解效果较好的HS-A32菌株进行了初步研究.提取该菌总DNA进行16S rDNA PCR扩增、测序,BLAST检索及序列分析表明,HS-A32菌与不动杆菌的同源性为98%.结合生理生化实验结果,初步确定HS-A32菌为不动杆菌属(Acinetobacter).通过薄层色谱(TLC)及高效液相色谱(HPLC)对MAP降解进行了定性和定量分析.结果表明,该菌能以MAP作为唯一的碳源和氮源生长.接种该菌于500mg/L的MAP无机盐液体培养基中,35℃、120r/min振荡培养3d,对甲胺磷降解率可达86%,是一株甲胺磷高效降解菌.Ten strains of meth ferent soils in which organop amidophos degrading bacteria were isolated from the difhosphates was used for a long period. The preliminary ding biochemical tests indicated that HS-A32 strain was belonged to Acinetobacter. This strain can use methamidophos as the sole carbon and nitrogen sources. The MAP degradation rate is up to 86% on the condition of 500 mg/L MAP, 35℃ and shaking cultured with 120 r/min. The residual MAP was analyzed by thin layer chromatography（TLC）. This method can be use in both MAP qualitative analysis and MAP separation and preparation, which has good sensitivity, high efficiency of separation and simply operation.

关 键 词：甲胺磷 降解菌 分离和筛选 16S rDNA薄层色谱 

分 类 号：X172[环境科学与工程—环境科学] Q939.9[生物学—微生物学]