C57BL/6J小鼠非胰岛素依赖性糖尿病模型研究  

Study of C57BL/6J mouse model of Non-Insulin-Dependent Diabetes Mellitus

作　　者：王兴[1] 闫智勇[1] 郝晓锋 

机构地区：[1]西南交通大学药学院,峨眉614202 [2]康弘集团.成都法玛基因科技有限公司博士后科研工作站,成都610036

出　　处：《中药药理与临床》2007年第1期79-81,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家863计划新药筛选技术平台研究(No2002AA2Z343E)资助

摘　　要：目的:建立与人临床发病机理相似的非胰岛素依赖型糖尿病动物模型。方法用高脂高糖饲料喂养3周龄C57BL/6J小鼠,3周后以125mg/kg剂量注射STZ,继续喂养4周后成模。结果喂养7周后高脂饲料-STZ组小鼠血糖浓度达到Ⅱ型糖尿病小鼠成模标准。结论高脂肪饲料和STZ是建立NIDDM模型所必须的,高脂饲料加STZ组小鼠的血糖升高缓慢,体重增加与对照组接近,摄食量与饮水量无明显增加,在造模结束时表现出高血糖和正常胰岛素水平,整个代谢变化过程缓慢,与人类NIDDM病人自然发病过程十分接近。Objective： To develop a NIDDM mouse model that has similar pathogenesis with human clinic. Methods： C57BL/6J mice at 3 weeks age were fed on diets in high fat and sugar, to be injected with STZ with 125mg/kg and kept on the same diets for the next 4 weeks. Results： The serum glucose concentration of high fat-STZ group reached the standard of NIDDM mouse. Conclusion： High fat diet and STZ are necessary to develop the model of NIDDM. The serum glucose concentration of high fat and STZ group rise slowly, the increase of body weight is similar to control group. Food intake and water intake have not obvious increase. It has high serum glucose concentration and normal insulin concentration. The whole metabolism changes very slowly, it is very close to the NIDDM patients.

关 键 词：糖尿病 非胰岛素依赖型 小鼠 模型 

分 类 号：R-332[医药卫生]