氢化物发生-原子荧光光谱法测定富硒麦芽中的硒含量  

Determination of the selenium content in selenium-enriched malt by hydride generation atomic fluorescence spectrometry

作　　者：赵洪进[1] 孙泉云[2] 刘佩红[2] 唐文红[2] 刘家国[1] 刘艳娟[1] 王小龙[1] 

机构地区：[1]南京农业大学畜禽营养代谢病研究室,江苏南京210095 [2]上海市畜牧兽医站,上海201103

出　　处：《粮油食品科技》2007年第2期40-42,共3页Science and Technology of Cereals,Oils and Foods

基　　金：教育部高校博士点基金(RFDP20020307022);国家"十五"科技攻关项目(2002BA574A-10-1-6)

摘　　要：建立了氢化物发生-原子荧光光谱法测定富硒麦芽中硒的分析方法。研究了仪器的工作条件、试剂对硒测定的影响,探讨了消化试剂对样品消化的影响和共存离子的干扰及消除。在优化的工作条件下,硒的检出限(σ=3)为0.167μg/L,相对标准偏差(RSD)为1.203%,线性范围为0-100μg/L。应用该方法测定了不同富硒麦芽中的硒含量为11.729-32.746μg/g,加标回收率为94.1%-101.6%。A method for the determination of selenium from selenium-enriched malt by hydride generation atomic fluorescence spectrometry（ HG - AFS） has been developed. The effects of the instrumental and chemical parameters on atomic fluorescence signal of selenium were discussed and optimum conditions were recommended. The effects of digest reagents on digestion of samples and the interference from foreign ions on selenium were briefly discussed , Under the optimum conditions, the detection limit for selenium was 0,167μg/L, the relative standard deviation was 1. 203%, and the liner ranger was 0 - 100μg/L, A total selenium content of 11. 729 - 32. 746μg/g was detected from selenium - enriched malt by this method, and the recovery rate is 94.1% - 101.6%.

关 键 词：氢化物-原子荧光光谱法 富硒麦芽 硒 

分 类 号：O657.31[理学—分析化学]