5个鸡种腺苷琥珀酸裂解酶(ADSL)基因外显子9 SNP及其与鸡肉肌苷酸含量的相关分析  

Correlation Analysis between Single Nucleotide Polymorphism of the ADSL Exon 9 and Inosine Monophosphate Acid Content in Five Chicken Breeds

作　　者：张学余[1] 束婧婷[2] 季从亮[2] 陈国宏[2] 韩威[1] 

机构地区：[1]中国农业科学院家禽研究所,扬州225003 [2]扬州大学畜牧兽医学院,扬州225009

出　　处：《畜牧兽医学报》2007年第3期237-240,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家"863"计划(2001AA243081);国家科技基础平台(2004DKA30450);江苏省科技计划(BK2004050;BZ20053200050)

摘　　要：根据腺苷琥珀酸裂解酶(Adenylosuccinate lyase,ADSL)基因外显子9的序列设计引物,用PCR-SSCP的方法对隐性白羽鸡、丝羽乌骨鸡、萧山鸡、白耳鸡、藏鸡进行了单核苷酸多态性分析,并检测到了多态性,表现为3种基因型,对两种纯合子进行直接测序,结果发现9 839位碱基发生了突变,由C突变为A,将核苷酸序列翻译成氨基酸序列后,发现突变后引起ADSL基因第273位氨基酸由Pro突变为Thr,为错义突变。经独立性检验,发现基因型频率和基因频率分布与品种有关,但进一步方差分析并没有显示出该位点的多态性与胸肌的IMP含量之间存在关联。This study was designed to investigate the single nucleotide polymorphism（SNP） of ADSL gene in various breeds, including Recessive White chickens, Silkies chickens, Xiaoshan chickens, Baier chickens and Tibetan chickens. Primers for exon 9 in ADSL gene were designed from the database of chicken genomic sequence and polymorphisms were detected by PCR-SSCP and DNA sequencing. Three genotypes were found among individuals within 5 chicken breeds and a mutation C→A at 9 839 nucleotide was found. It was a nonsynonymous mutation, resulted in amino acid sequence at 273 change accordingly from Pro to Thr. The results x^2 showed that the frequency of genotype and the frequency of gene were significant different among breeds. However, The results from analysis of variation suggested that there was no significant relationship between this SNP site and IMP content in chickens.

关 键 词：鸡 腺苷琥珀酸裂解酶基因 单核苷酸多态性 肌苷酸 

分 类 号：S831.2[农业科学—畜牧学]