应用MSP方法检测非小细胞肺癌RASSFlA基因启动子甲基化  

Analysis of Methylation Status of RASSFlA Promoter Region and Transcription Level of Downstream Genes in Non-Small Cell Lung Cancer

作　　者：于正洪[1] 王玉才[1] 季洪赞[2] 高大志[3] 王苏莉[4] 

机构地区：[1]南京军区南京总医院肿瘤内科,江苏南京210002 [2]南京军区南京总医院消化内科,江苏南京210002 [3]南京军区南京总医院医学影像科,江苏南京210002 [4]南京军区南京总医院保健办,江苏南京210002

出　　处：《现代检验医学杂志》2007年第1期15-17,共3页Journal of Modern Laboratory Medicine

摘　　要：目的探讨应用甲基化特异性PCR(MSP)方法检测非小细胞肺癌(NSCLC)RASSFlA基因启动子甲基化情况。方法留取58例非小细胞肺癌患者手术标本及正常肺组织,甲基化特异性PCR分析RASSFlA基因启动子甲基化情况。结果58例非小细胞肺癌中RA SSFlA基因启动子甲基化阳性率为34.5%,甲基化与各临床参数之间无显著相关性。结论原发性非小细胞肺癌中存在着较高比例的RASSFlA启动子过度甲基化,提示RASSFlA在非小细胞肺癌的发生中起着重要作用。Objective To detect the methylation status of RASSF1A promoter region in non-small cell lung cancer by using methylation-specific PCR technique （MSP）. Methods 58 resected non-small cell lung cancer and corresponding normal lung tissue sample were collected. Methylation specific PCR was conducted to determine the methylation status of RASSF1A promoter. Results Methylation of RASSF1A promoter was detected in 20（34.5%）cancer samples,while no significant correlation between the methylation status and clinical factors was found. Conclusion Frequent hypermethylation of RASSF1A promoter exist in primary non-small cell lung cancer,suggesting a multifunctional role of RASSF1A in the genesis of non-small cell lung cancer.

关 键 词：肺癌 RASSF1A 甲基化 

分 类 号：R730.43[医药卫生—肿瘤] R734.2[医药卫生—临床医学]