肠化胃黏膜病变组织HSP60的表达与幽门螺杆菌感染的相关性  

Expression of heat shock protein 60 in gastric mucosa with intestinal metaplasia and its correlation with Helicobacter pylori infection

作　　者：刘东屏[1] 吕晓辉[1] 张艳梅[1] 王炳元[1] 

机构地区：[1]中国医科大学附属第一医院消化内科,辽宁省沈阳市110001

出　　处：《世界华人消化杂志》2007年第7期758-761,共4页World Chinese Journal of Digestology

摘　　要：目的:探讨HSP60在肠化胃黏膜组织中的表达及与Hpylori感染之间的关系.方法:对171例肠化胃黏膜组织其中Ⅰ型肠化54例、Ⅱ型肠化76例、Ⅲ型肠化41例,对照组浅表性胃炎40例,胃癌49例,采用SP免疫组织化学技术进行胃黏膜HSP60的检测,用ELISA,H pylori IgG抗体检测及H pylori-DNA PCR方法进行H pylori感染情况的判定.结果:HSP60在肠化胃黏膜组织中的表达主要为中等强度阳性,Ⅰ,Ⅱ,Ⅲ型肠化胃黏膜组织的阳性率分别为35.19%,51.32%,75.61%,过表达率分别为1.9%,3.9%和19.5%;浅表性胃炎组织中的表达多为弱阳性或中等强度阳性,阳性表达率为30.00%,与浅表性胃炎相比肠化胃黏膜组织中HSP60的表达显著增加(P<0.01);HSP60在胃癌组织的表达以强阳性和中等强度阳性为主,阳性表达率为73.47%,过表达率为34.69%,明显高于Ⅰ,Ⅱ型肠化胃黏膜组织中的表达,与Ⅲ型肠化胃黏膜组织相比差异不显著(P>0.05),肠化胃黏膜组织的H pylori感染和HSP60表达有明显的相关性(r=-0.191,P=0.012).结论:HSP60的过度表达发生在胃黏膜病变的进展过程中;肠化胃黏膜组织中Hpylori感染和HSP60表达有明显的相关性.AIM： To investigate the expression of heat shock protein 60 （HSP60） and H pylori infection in gastric mucosa with intestinal metaplasia （IM）. METHODS： A total of 171 IM mucosa （54, 76, and 41 cases for type Ⅰ, Ⅱ, and Ⅲ, respectively） and 89 controls （40 and 49 cases for superficial gastritis and gastric cancer, respectively） were included in this study. SP immunohistochemisty was used to detect the expression of HSP60 and H pylori infection was judged by enzyme-linked immunosorbnent assay （ELISA）, anti-H pylori IgG detection and H pylori -DNA polymerase chain reaction （PCR）, respectively. RESULTS： HSP60 expression was moderately positive in gastric mucosa with type Ⅰ and Ⅱ and Ⅲ IM; the positive rate was 35.19% and 51.32% and 75.61%, and the over-expression rate was 1.9%, 3.9% and 19.5%, respectively. HSP60 expression was weak or moderately positive in superficial gastritis, and the positive rate was 30.00%. The positive rate of HSP60 expression in IM mucosa was increased statistically as compared with that in superficial gastritis （P 〈 0.01）. Moderately or strongly positive expression of HSP60 was predominant （73.47%） in gastric cancer, and the over-expression rate was 34.69%, which was markedly higher than that in type Ⅰ and type Ⅱ IM. There was a negative relationship between HSP60 expression and H pylori infection of IM mucosa （r = -0.191, P = 0.012）. CONCLUSION： HSP60 over-expression occurs in the progression of gastric mucosal lesions, and H pylori infection of IM mucosa is associated with HSP60 expression.

关 键 词：热休克蛋白60 肠化胃黏膜 胃癌 幽门螺杆菌 

分 类 号：R573[医药卫生—消化系统]