一株传染性软化病病毒的分离和鉴定  

Isolation and Identification of an Infectious Flacherie Virus from Silkworm,Bombyx mori in Zhejiang Province of China

作　　者：陆奇能[1] 朱宏杰[1] 洪健[2] 金伟[1] 张凡[1] 鲁兴萌[1] 

机构地区：[1]浙江大学动物科学学院无脊椎动物病理学研究室,浙江杭州310029 [2]浙江大学分析测试中心,浙江杭州310029

出　　处：《病毒学报》2007年第2期143-147,共5页Chinese Journal of Virology

基　　金：浙江省科学技术厅重点科研项目(2003C22013)

摘　　要：病毒性软化病是一种严重影响蚕业生产的流行病，其病原为家蚕传染性软化病病毒（Bombyx mori infectiousflacherie virus，BmIFV）。本实验室在浙江省收集到具有软化病症状的家蚕幼虫，并分离纯化获得一株病毒。电镜观察到病毒粒子为直径26nm左右、无包涵体的球状颗粒。病毒添食四龄起蚕和五龄起蚕，分别在3～4d和5～7d出现明显症状和病变。纯化病毒SDS-PAGE条带与BmIFV蛋白相仿。病毒核酸鉴定为RNA，并可用套式RT-PCR扩增出特异性片段，测序发现片段序列和BmIFV日本坂城株的同源性为99．5％。由此可确定该病毒为BmIFV，这是我国首次分离到该病毒，暂命名为BmIFV-CHN001。Infectious flacherie virus（IFV） is the agent causing flacherie disease of silkworm. We collected the silkworms with suspective symptom in Zhejiang Province and a virus was isolated. Electron microscopy revealed the non-enveloped global particles that measured approximately 26nm in diameter. Feeding fourth instar and fifth instar with virus-smeared leaves, pathological changes were observed after 3-4d and 5-7d respectively. SDS-PAGE of purified virions showed resemblance to the typical protein bands of IFV. Genomic nucleic acid of the virus was identified to be RNA, when subjected to nested RT-PCR, specific amplified fragments were obtained. Subsequent analysis indicated the sequence of the virus shared a 99.5% similarity with the standard strain of IFV. It was the first IFV case discovered in China and the strain isolated was named BmlFV-CHN001.

关 键 词：家蚕 传染性软化病病毒 分离鉴定 

分 类 号：S884.5[农业科学—特种经济动物饲养]