甲硫腺苷磷酸化酶在人支气管上皮细胞辐射致癌模型中的表达变化研究  

Downregulation of Methylthioadenosine Phosphorylase (MTAP) In Malignant BERP35T-2 cells exposed upon α particles

作　　者：李邦印[1] 张开泰[1] 霍艳英[1] 胡迎春[1] 徐勤枝[1] 应万涛[1] 吴德昌[1] 

机构地区：[1]解放军总医院第二附属医院

出　　处：《临床肺科杂志》2007年第4期321-324,共4页Journal of Clinical Pulmonary Medicine

基　　金：国家重点基础研究发展规划973项目(批准号G1998051207)基金资助

摘　　要：目的研究甲硫腺苷磷酸化酶(Methylthioadenosine Phosphoylase,MTAP)在人支气管上皮细胞(BEP2D)辐射致癌恶性转化前后的表达水平变化。方法培养永生化细胞BEP2D和恶性转化细胞BERP35T-2;制备细胞总蛋白质,进行双相电泳和质谱分析;选择有表达差异的蛋白质点MTAP,在细胞模型中进行Northern Blot和Western blot分析。结果双相电泳发现MTAP在恶性转化细胞BERP35T-2中基本没有表达;Northern Blot和Western Blot分析表明MTAP基因在BEP2D永生化细胞中转录和翻译表达水平均很高,而在BERP35T-2恶转细胞中表达极低;发现MTAP基因在两种细胞中的表达差别在10倍以上。结论MTAP的低表达与辐射致肺癌的发生有密切关系,是BEP2D受到α粒子辐射后基因表达谱的主要改变之一。Objective MTAP （methyhhioadenosine phosphorylase） is an enzyme in the salvage celluar adenine and methionine synthesis, which is frequently codeleted with p16INK4A referenced by lots of researches in Occidental tumors. The aim of this study was to investigate the expression alteration of MTAP gene in tumorgenesis of bronchial epithelial cell line. Experimental design ： Total proteins from BEP2D and BERP35T-2 cells was extracted and analysed by 2-D electrophoresis and peptide mass fingerprinting spectrum. Northern blot Western blot was performed to confirm differences of MTAP gene expression between BEP2D and BERP35T-2. Results By proteome analysis, MTAP was highly expressed in BEP2D cell lines, but no expression was detected in BERP35T-2. Through Northern blot and Western blot analysis,MTAP was proved to be expressed in a much lower level in BERP35T-2 than that in BEP2D cells. Conclusion These results indicate that the downregulation of MTAP gene might contribute to the malignancy of bronchial epithelial cells, and MTAP might act as a candidate of tumor suppressor genes.

关 键 词：甲硫腺苷磷酸化酶 BEP2D Α粒子 

分 类 号：R734.2[医药卫生—肿瘤]