Isolation and optimization of production of Astaxanthin from Antarctic yeast Rhodotorula sp. NJ298  

作　　者：刘均玲 Miao Jinlai Sun Xiuqin Wang Quanfu Li Guangyou 

机构地区：[1]College of Life Sciences, Ocean University of China, Qingdao 266003, P.R. China [2]The Ocean College of Hainan University, Haikou 570228, P.R. China [3]Key Laboratory of Marine Bio-active Substances, First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, P.R. China

出　　处：《High Technology Letters》2007年第1期103-108,共6页高技术通讯（英文版）

基　　金：Supported by the National Natural Science Foundation of China （No. 40406003 and 40206022）.

摘　　要：Rhodotorula sp. NJ298 which could produce carotenoids was isolated from Antarctic sea ice. The major carotenoid was identified as astaxanthin by Liquid Chromatography/Mass Spectrometry （LC/MS）, and its content accounted for 87.62% of total carotenoids （1,786 μg/g）. High Performance Liquid Chromatogrephy （HPLC） analysis showed that the purity of the astaxanthin reached about 96. 16% through a simple purification. Maximum astaxanthin production （1,908μg/g） was obtained when the yeast was grown at 10 ℃ in seawater medium containing 5 g/L sodium acetate, 5 g/L peptone, 0.5 g/L NaC1, 0.01 g/L KH2PO4; 0.01 g/L MgSO4·7H20 and 0.001 g/L FeSO4·7H20 at pH 7.5.

关 键 词：antarctic yeast carotenoids ASTAXANTHIN ISOLATION optimization 

分 类 号：TQ926.4[轻工技术与工程—发酵工程] TQ611.3