小球藻异养生长及叶黄素合成量影响因子的优化研究  

Optimization of Culture Parameters for Heterotrophic Cultivation of Chlorella pyrenoidosa and Lutein Production

作　　者：吴正云[1] 曲春波[1] 史贤明[1] 

机构地区：[1]上海交通大学食品科学与工程系,陆伯勋食品安全研究中心,上海201101

出　　处：《上海交通大学学报（农业科学版）》2007年第1期6-11,共6页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：国家863计划资助项目(2006AA02Z226)

摘　　要：通过摇瓶实验研究了影响小球藻异养生长和叶黄素合成的6个重要因子:接种量、通气量以及葡萄糖、KNO3、KH2PO4和MgSO4的起始浓度,并通过均匀设计实验建立了4种主要培养基组分浓度对细胞生长和叶黄素合成量影响的数学模型。研究表明:在摇瓶实验中,培养液装量少或转速快有利于小球藻细胞生长和叶黄素合成,因此充足的通气量是必要条件;在10～60g·L-1范围内,较高的葡萄糖起始浓度可提高小球藻的最终生物量和叶黄素产量,但抑制小球藻的细胞生长;在2∶1～12∶1范围内,较低的C∶N比有利于细胞中叶黄素含量的增加,而对小球藻细胞生长的影响较小。通过建立数学模型、对上述参数进行优化,小球藻的比生长速率和细胞中叶黄素含量得到了显著提高,最大比生长速率和细胞中叶黄素含量分别达到2.32d-1和3.18mg·L-1。The effects of six culture parameters including inocula, aeration, initial concentrations of glucose, KNO3, KH2PO4 and MgSO4 on both the heterotrophic growth of Chlorella pyrenoidosa and lutein production were investigated based on flask experiments. Mathematic models were developed by means of uniform design to describe the effects of four medium components on cell growth and cellular lutein content. It was found that lower medium volume or higher agitation rate in flask experiments favored both cell growth and lutein formation, which means that sufficient aeration is the necessity. In the range between 10-60 g·L^-1, higher initial concentration of glucose resulted in higher f＇mal biomass concentration and lutein yield, but inhibited the cell growth. In the range between 2：1-12：1, lower C：N ratio increased cellular lutein content but had less effects on the cell growth. Significant elevations of the specific growth rate of Chlorella and cellular lutein content were obtained by model-based optimization. The highest specific growth rate and cellular lutein content attained as 2.32 d^-1 and 3.18 mg·L^-1, respectively.

关 键 词：小球藻 异养培养 叶黄素 均匀设计 优化 

分 类 号：TS2[轻工技术与工程—食品科学与工程]