血-脑屏障和血-神经屏障内皮细胞的比较研究  

Difference of Endothelial Cell in Ultrastructure,Markers and Permeability between Blood-Brain Barrier and Blood-Nerve Barrier

作　　者：陈海波[1] 陶沂[1] 尹岭[2] 丁素菊[1] 刘式威[2] 杜侃[2] 

机构地区：[1]第二军医大学长海医院神经内科,上海市200433 [2]解放军总医院神经信息中心,北京市100853

出　　处：《中国康复理论与实践》2007年第3期238-239,F0003,共3页Chinese Journal of Rehabilitation Theory and Practice

摘　　要：目的研究血-脑屏障与血-神经屏障内皮细胞在超微结构、通透性及标记物表达方面的差异。方法取大鼠前额叶皮层和坐骨神经。用电镜观察内皮细胞的超微结构;用免疫组织化学的方法检测转铁蛋白受体(OX-26)和内皮屏障抗原(EBA)的表达及微血管周围纤维蛋白原外渗。结果电镜显示,血-脑屏障和血-神经屏障微血管均为紧密连接,但血-神经屏障吞饮小泡较血-脑屏障多(P<0.05)。免疫组织化学显示,血-神经屏障OX-26和EBA阴性表达,微血管周围可见微量纤维蛋白原外渗;血-脑屏障OX-26和EBA均强阳性表达,微血管周围未见纤维蛋白原外渗。结论血-脑屏障与血-神经屏障内皮细胞虽然均为紧密连接,但两者在吞饮小泡数量、标记物表达及通透性等方面均存在差异。Objective To investigate the difference of endothelial cell （EC） in ultrastructure, markers and permeability between blood-brain barrier （BBB） and blood-nerve barrier （BNB）. Methods The rat brain cortex and sciatic nerve were removed. Ultrastructure of endothelial cell was observed with electron microscopy. Employing immunohistochemical （IHC） staining, the relative distribution was determined for the OX-26, endothelial barrier antigen （EBA） and extravasated fibrinogen around microvessels. Results Microvessels both in BBB and in BNB did share the same tight junctions. However, BNB had a significantly larger number of vesicles than that of BBB. Microvessels in BNB did not express the OX-26 and EBA, but staining weakly positive for fibrinogen, while microvessels in BBB showed strong positive for OX-26 and EBA, perivascular staining of fibrinogen had not been seen. Conclusion Tight junctions were present in both BBB and BNB endothelial cell, but there was a significant difference between them in the number of the pinocytotic vesicles, markers expression and permeability.

关 键 词：血-脑屏障 血-神经屏障 超微结构 免疫组织化学 

分 类 号：R741.02[医药卫生—神经病学与精神病学]