大鼠坐骨神经切断模型脊髓背角内PKCγ的变化(英文)  

PAIN-RELATED INCREASES OF PKCγ IN RAT SPINAL DORSAL HORN FOLLOWING SCIATIC NERVE AXOTOMY

作　　者：倪同上[1] 梅光东[1] 蒋正尧[1] 

机构地区：[1]青岛大学医学院生理学教研室,山东青岛266021

出　　处：《青岛大学医学院学报》2007年第1期31-33,共3页Acta Academiae Medicinae Qingdao Universitatis

摘　　要：目的探讨大鼠坐骨神经切断模型脊髓背角内PKCγ的变化及意义。方法建立大鼠坐骨神经切断模型，在坐骨神经切断后2、5、10、15、20、30、40、60d，取脊髓背角，应用免疫组织化学方法进行染色，图像分析软件测量并比较脊髓背角手术侧和对照侧的免疫强度。结果从第2天开始，手术侧脊髓背角L4～L5节段PKCγ免疫阳性反应较对照侧明显增强，差异有显著性（t=3．12，P＜0．05）；在第15天时免疫阳性反应最强，是对照组的1．89倍（t=4．85，P＜0．01），然后逐渐减弱，2个月后恢复到正常水平（t=0．91，P＞0．05）。结论PKCγ在由神经损伤引起的脊髓背角神经元兴奋性改变中发挥重要作用。Objective To investigate the pain-related changes and possible function of protein kinase C7 （PKC7） in the rat spinal dorsal horn following sciatic nerve axotomy. Methods The density of PKC7 immunoreactivity in the dorsal horn following sciatic nerve transection was valued in different time after surgery, 2,5,10,15,20,30,40, and 60 days later respectively. The value （light absorbance value） was analyzed by contrasting the contralateral side and ipsilateral side with a computer-assisted ima- ging analysis system. Results Axotomy of sciatic nerve significantly increased PKC7 immunoreactivity in the contralateral superficial dorsal horn of the L4 and L5 segments two to 40 days after the transection. Two days later, immunoreactivity in contralateral side had changed significantly （t=3.12,P〈0.05）. This value reached 1.89 times highest in contralateral side 15 days later （t=4.85 ,P〈0.01）, while after a period of two months it returned to the normal level （t=0.91 ,P〉0.05）. Conclusion PKC7 plays an important role in the changes of excitability of dorsal horn neurons caused by nerve injury.

关 键 词：脊髓背角 蛋白激酶C 坐骨神经 免疫组织化学 大鼠 

分 类 号：R361[医药卫生—病理学]