结肠癌原发灶及其淋巴结转移灶中E钙粘素和β连环素表达  

Expression of E-cadherin and β-catenin in Primary Lesion with Colon Carcinoma and the Metastatic Lymphonodes

作　　者：谷彦军[1] 张诗武[2] 宋立新[1] 魏焕萍[1] 刘亚敏[1] 何瑞波[1] 

机构地区：[1]武警医学院病理教研室,天津300162 [2]天津市肿瘤医院,天津300060

出　　处：《中国肿瘤》2007年第2期127-130,共4页China Cancer

摘　　要：[目的]探讨结肠癌原发灶及其淋巴结转移灶中E钙粘素(E-cad)和β连环素(β-cat)表达的临床意义。[方法]采用组织芯片技术,应用免疫组化方法检测81例结肠癌原发灶及其淋巴结转移灶组织中E-cad和β-cat的表达。[结果]原发灶及其淋巴结转移灶中β-cat的正常表达率分别为23.46%和4.94%,原发灶中显著高于转移灶(P<0.01),尤其是高中分化病例,转移灶中异位表达率显著高于原发灶(45.68%比28.40%,P<0.01),尤其是管状腺癌;原发灶中β-cat表达强度显著高于转移灶(P<0.01)。E-cad的表达强度在原发灶显著高于转移灶(P<0.01),尤其是管状腺癌和高中分化病例。[结论]低表达E-cad和β-cat及异位表达β-cat的结肠癌细胞可能具有较强的转移能力,更易于发生淋巴道转移。[Purpose]To explore the clinical significance of expression of E-cadherin and β-catenin in both primary lesion with colon carcinoma and the metastatic lymphonodes. [Methods] Immunohistochemical staining of E-cadherin and β-catenin with tissue microarrays was carried out in 81 cases with colorectal carcinoma, including primary lesion and the metastatic lymphonodes. [Results ] Expression rate of β-catenin in primary lesion and metastatic nodes was 23.46% and 4.94%, respectively. The rate in metastatic lesion was significantly lower than that in primary lesion （P〈0.01）, especially that in well and moderately differentiated carcinoma. The extopic expression rate in metastatic carcinoma was significantly higher than that in primary lesion （45.68% vs. 28.40%,P〈0.01） ,especially in tubiforrn adenocarcinoma. Expression of β-catenin in metastatic carcinomas was significantly lower than that in primary colon carcinomas cinoma was significantly lower than that in primary colon （P〈0.01）. Expression of E-cadherin in metastatic carcarcinoma（P〈0.01） ,especially in tubiforrn adenocarcinoma and well and moderately differentiated carcinoma. [Conclusions] Colon cancer cells with low expression of E-cadherin and β-catenin, and extopic expression of β-catenin are more invasive and likely to lymphonglaudula metastasis..

关 键 词：结肠肿瘤 E钙粘素 Β连环素 组织芯片 免疫组织化学 

分 类 号：R735.35[医药卫生—肿瘤]