固相法合成多肽AN-M的工艺研究  

Study on Artwork of Fmoc Solid-Phase Synthesis of AN-M

作　　者：任付平[1] 朱立国[1] 陈五岭[1] 

机构地区：[1]西北大学生命科学学院,陕西西安710069

出　　处：《现代生物医学进展》2007年第1期25-27,共3页Progress in Modern Biomedicine

摘　　要：目的:探讨生物活性肽AN-M的固相合成工艺,并为工业化合成目的肽提供理论依据。方法采用固相法,以Fmoc-保护基保护的α-氨基酸和Wang树脂为原料,经1—氧—3—双二甲胺羧基苯骈三氮唑四氟化硼盐(TBTU)、1—羟基苯并三氮唑(HoBt)、二异丙基乙胺(DIEA)缩合,20%哌啶的DMF溶液脱保护,用切割试剂将AN-M粗品从Wang树脂上切割下来。结果经反相高效液相色谱分析纯化,可得目的肽的得率大于67．00%,最终成品的纯度在98．78%以上,经质谱鉴定其分子量与理论值一致。结论该合成方法步骤简便、便于操作、产品得率高,可用于大规模合成目的肽。Objective： To study the artwork of solid phase synthesis of biologically active peptide AN-M, and to provide the theoretical basis for industrialization synthesis of objective peptide. Methods： Using Solid-Phase synthesis method, based on the materials of a-amino acids protected by Fmoc-protecting group and Wang resin, after condensation and cleaved by HoBt/ DIEA and TFA-H20, the crude products of polypeptide AN-M were cut down from the Wang Resin. Results： By the analysis and purification of RP-HPLC, the product pick-up rate of objective peptide was more than 67%, and the purity of finished product was over 98.78%, whose molecular weight was identical with theoretical value by mass--spectrum identification. Conclution： This synthesis method is simple and easy to operate, with high product pick-up rate, which can be applied to the synthesizing of objective peptide on a large scale.

关 键 词：Fmoe 固相合成法 多肽 AN-M 

分 类 号：TQ406.31[化学工程]