水貂源犬瘟热病毒F基因真核表达载体的构建及鉴定  

Construction and Identification of Eukaryotic Expression Plasmid with Fusion Protein Gene of Canine Distemper Virus Isolated from Mink

作　　者：苏凤艳[1] 全学俊 宗春苗[1] 王卓聪[1] 丁庆东[1] 王全凯[1] 

机构地区：[1]吉林农业大学中药材学院,长春130118 [2]图门市牧业管理局,延吉130042

出　　处：《经济动物学报》2007年第1期23-26,共4页Journal of Economic Animal

摘　　要：通过T-A克隆技术,成功地构建了克隆载体PMD-18T-CDVF,用KpnI和BamHI双酶切克隆质粒PMD-18T-CDVF后,回收F基因,并将此基因定向克隆至相同双酶切回收后的pcDNA3.1真核表达载体中,获得重组质粒pcDNA3.1-CDVF。经DNA测序、限制性内切酶分析和PCR鉴定,证实成功地构建了重组质粒,从而为研究犬瘟热新型疫苗奠定了基础。The clone vector PMD-18T-CDVF with fusion gene of canine distemper virus isolated from a mink was successfully constructed by T- A clone technique and was digested with restriction enzyme of Kpn I and BamH I. The F gene was cloned into eukaryotic expression vectorpcDNA3.1. Finally a recombinant plasmid named pcDNA3.1-CDVF was constructed and identified by PCR, restriction enzyme analysis and sequencing.

关 键 词：水貂 犬瘟热病毒 F基因 真核表达载体 

分 类 号：Q78[生物学—分子生物学]