早期糖尿病大鼠视网膜蛋白质表达变化的初步研究  

Analysis of expression of retina protein in early diabetic rats

作　　者：刘尚清[1] 张艳艳[2] 羊惠君[1] 谢贤镛[3] 胡为民[4] 袁爱花[1] 

机构地区：[1]四川大学基础与法医学院人体解剖学与组织胚胎学教研室,成都610046 [2]川北医学院电镜室,南充637007 [3]川北医学院病理学教研室,南充637007 [4]川北医学院免疫学与分子生物学研究所,南充637007

出　　处：《眼科研究》2007年第3期186-189,共4页Chinese Ophthalmic Research

摘　　要：目的观察2个月病程大鼠视网膜组织蛋白质变化情况;建立并优化视网膜蛋白质组分析所需要的双向凝胶电泳(2-DE)技术,提高其分辨率及重复性。方法提取视网膜蛋白质进行2-DE,对影响2-DE结果的各种因素进行调整、优化。用PDQUest7.3.2软件分析凝胶图谱以获得差异表达的蛋白点。结果成功获得了视网膜组织的2-DE图谱。比较后得到36个表达差异有统计学意义(P<0.05)的蛋白质点,包括上调5个,下调23个,消失8个。结论2个月病程糖尿病大鼠视网膜已经有蛋白质表达的差异。已建立的视网膜蛋白质2-DE技术将为进一步开展视网膜疾病的蛋白质组学研究奠定基础。Objective Diabetic retinopathy is a complication of diabetes and often results in blindness. The aim of the research was to observe the proteome changes of retinal tissues in diabetic rats, and establish and optimize a two-dimensional polyacrylamide gel electrophoresis （2-DE） technique for retina proteomics. Methods The rats （30 healthy adult female SD rats） in diabetic group received a single intraperitoneal injection of Alloxan Tetrahydrate （200 mg/kg）, and the rats in the control group （10） received injection of equal volume of normal sodium alone. The rats with urine glucose strongly positive and blood glucose levels over 16.7 mmol/L were deemed diabetes models. The alterations of protein expression in retinal tissue from normal and 8-week diabetic rats were observed using 2-DE. Various conditions of retina proteomic 2-DE were adjusted, optimized and protein spots of differential expressions were obtained through analysis of 2-DE images with PDQuest 7.3.2 software. Results Using the technique stated above, satisfactory 2-DE maps of retina were obtained and a repeatable 2-DE technique was established. Thirty-six protein spots exhibited statistically significant in the retinas of diabetic rats, which included 5 proteins upregulation,23 proteins down-regulation, and 8 of them were disappeared in comparison with control tissue. Conclusion The differences of protein expression in retina were observed between diabetic and normal rats. Our established 2-DE technique of retina proteins analysis can be effectively applied in the study of proteomics of retina diseases.

关 键 词：糖尿病 大鼠 视网膜 蛋白质表达 双向凝胶电泳 

分 类 号：R774.1[医药卫生—眼科]