裸燕麦麸皮中β-谷甾醇的提取  

Study of the Extraction of β-sitosterol in Bare Oat Bran

作　　者：乌汉其木格[1] 张美莉[1] 

机构地区：[1]内蒙古农业大学食品科学与工程学院

出　　处：《食品与发酵工业》2007年第1期131-133,共3页Food and Fermentation Industries

摘　　要：采用溶剂结晶法从裸燕麦麸皮中提取β-谷甾醇,用重结晶法进一步精制,并用薄层层析法(TLC)分析鉴定,用紫外分光光度法测定其β-谷甾醇含量。研究结果表明:体积分数为95%的乙醇作为提取剂时非皂化物与提取剂的最佳料液比为1∶20(g∶mL),β-谷甾醇提取物在G型硅胶TLC板上展开后斑点与标准样品斑点平行,标准样品的Rf值为0.272,提取物的Rf值为0.268。在最佳料液比下提取之后,提取物中β-谷甾醇平均含量为2.02%。The extraction of β-sitosterol in bare oat bran was studied. β-sitosterol was extracted from bare oat bran by re- crystallization. Thin layer - chromatography was used to identify the β-sitosterol. The content of β- sitosterol was determined by ultraviolet spectrophotometer. The result showed that when CH3CH2OH （95 % ） was used to extract β-sitosterol , the best ratio was 1：20 （g/mL） . The extracts have parallel spot with β-sitosterol sample on silica gel plates . The Rf of β-sitosterol sample was 0. 272 and the Rf of extracts was 0. 268 . The average content of β-sitosterol in extract was 2.02 % .

关 键 词：裸燕麦麸皮 Β-谷甾醇 提取 

分 类 号：TS210.9[轻工技术与工程—粮食、油脂及植物蛋白工程]