翼状胬肉纤维组织起源于间充质干细胞的证据  

Evidence of Fibrous Tissue in Pterygium Originated from Mesenchymal Stem Cells

作　　者：李永平[1] 朱哲[1] 张文忻[1] 刘琳 梁丹[1] 

机构地区：[1]中山大学中山眼科中心//眼科学国家重点实验室,广东广州510060 [2]广州陆军总医院,广东广州510120

出　　处：《中山大学学报（医学科学版）》2007年第2期127-130,136,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家自然科学基金项目(30672276);广东省社会发展攻关项目(2003A3020302);广州市科委领域专项资助项目(2004B30901011)

摘　　要：【目的】研究翼状胬肉组织病理学特征,探讨多能干细胞在翼状胬肉发生过程中的作用。【方法】对218例手术切除翼状胬肉标本,进行形态学、免疫组织化学和免疫荧光共聚焦显微镜观察其形态学变化及CD34、波形纤维蛋白(VIM)、平滑肌肌动蛋白(SMA),S-100在翼状胬肉中的表达。【结果】纤维组织增生及新生血管形成为翼状胬肉的主要病理改变。增生的纤维组织不同区域变化不一,主要呈现两类变化:一为排列致密,类似巩膜纤维组织;另外就是疏松区:仅见一些梭形、多角形、星状具有一些突起的纤维母细胞样的细胞,疏松排列,之间无明显的胶原纤维存在。免疫组织化学和免疫荧光显示一些增生活跃的纤维母细胞明显表达CD34,成熟的纤维组织内的纤维细胞CD34则为阴性。此外,218例中56例显示了平滑肌的存在,44例有脂肪组织。【结论】翼状胬肉组织中的纤维组织源自于间充质干细胞,并可向平滑肌及脂肪组织分化。[Objective] To investigate the histopathological feature of pterygium and the significance of multipotent stem cells in pterygium. [Methods ] A total of 218 cases of pterygium were collected after the clinic operation, and these specimen were studied under the light microscope by HE, PAS, stain and confocal scanning laser ophthalmoscope by immunofluorescent staining with CD34, VIM, VEGF, SMA, S-100. [Results] Fibroplasia and neovascnlarization were the main changes in pterygium. Fibroplasia varied in different region, two main presentation were observed： First, the tissue arrange compactly like the scleral fibre; Second, in some ramified and loose region, a few of spindle-shape, polygonal, asteroid fibroblast-like cells could be seen only, and arranged loosely. No apparent collagen fibers were identified between them. lmmunohistochemistry and illustrated that CD34 were positively detected in some region where the fibroblast actively proliferated, but the fibrocyte in mature fibrous tissue were negatively expressed. Besides, 56 cases had smooth muscle and 44 cases had adipocyte in pterygium. [Conclusion] The fibrous tissues in pterygium originated from mesenchymal stem cells ,they can differentiate into smooth muscle and adipose tissue.

关 键 词：翼状胬肉 病理学 间充质干细胞 纤维组织 细胞起源 

分 类 号：R77[医药卫生—眼科]