3种化学物质诱导马铃薯块茎抗早疫病的初步研究  

Preliminary Research on Inducing Resistance in Potato Tuber Against Early Blight with 3 Chemicals

作　　者：高冬婷[1] 蒋继志[1] 刘洋[1] 王凤春 范继业[3] 边瑞环[4] 

机构地区：[1]河北大学生命科学学院,河北保定071002 [2]河北省燕山科学试验站,河北唐山064300 [3]河北化工医药职业技术学院制药工程系,河北石家庄050000 [4]河北农业大学理学院,河北保定071000

出　　处：《华北农学报》2007年第1期148-151,共4页Acta Agriculturae Boreali-Sinica

基　　金：河北省自然科学基金项目(300081);河北省科学技术厅项目(96Z1102)

摘　　要：利用苯酚、对氨基苯甲酸和苯甲酸等3种芳香族类化合物作为激发子,对马铃薯块茎抗早疫病的效果进行了探讨,同时分析了诱导处理后马铃薯块茎中与抗病性相关的酶活性的变化。结果表明,供试的3种化合物均在较低浓度下具有较强的诱导抗病作用,苯酚、苯甲酸和对氨基苯甲酸分别在1,0.01和5 mmol/L时诱导的保护率最高,分别达到43.21%,40.72%和28.75%,均与对照差异极显著。经上述浓度的3种化合物诱导处理后的马铃薯块茎中,过氧化物酶(POD)和苯丙氨酸解氨酶(PAL)的活性均有大幅度上升,明显高于对照,表明这些芳香族类化合物可能通过增加马铃薯体内POD和PAL的活性来发挥其诱导抗病作用。Phenol, benzoic acid, and paraaminobenzoic acid, as the elicitors, were evaluated in lab of their efficiences to induce potato tuber against early blight, and the change of resistance related enzymes were investgated in induced potato tuber. The results indicated that 3 chemicals could greatly enhance resistance of potato tuber to early blight at low concentrations. Protection rates induced by phenol （1 mmol/L）, benzoic acid （0. 01 mmol/L）, and paraaminobenzoic acid （5 mmol/L） were 43.21%, 40.72 %, and 28.75 %, respectively. There were marked diffrences at 0.01 level comprison with control. The activities of peroxidase （POD） and phenylalanine （PAL） related to systemic resistance increased great extent in induced tubers comparing with the control. It seemed that the 3 chemicals could enhance resistance of tuber via activating POD and PAL.

关 键 词：化学物质 马铃薯 过氧化物酶 苯丙氨酸解氨酶 

分 类 号：S532.01[农业科学—作物学]