四种中成药对气血双虚模型小鼠血象及免疫水平的影响  

Effects of four Chinese medicines on the immunity of mice with deficiency in both Qi and blood

作　　者：苗明三[1] 周立华[1] 侯江红[1] 陈纲领[1] 张玉林[1] 

机构地区：[1]河南中医学院艾滋病研究所,河南省郑州市450000

出　　处：《中国组织工程研究与临床康复》2007年第11期2025-2028,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家"十五"省部联动项目(2004BA719A13-06);国家"十五"科技攻关艾滋病专项(2004BA719A09-0204)~~

摘　　要：目的:为艾滋病抗病毒疗法所致的骨髓不良反应筛选疗效确切的中成药,观察分析参芪颗粒、复方阿胶浆、贞芪扶正颗粒、复方皂矾丸四种中成药对放血和注射环磷酰胺联合复制的气血双虚模型小鼠血象及免疫水平的影响。方法:实验于2005-08/09在河南中医学院药理实验室完成。①参芪颗粒(江西山高制药有限公司生产,批号040702);复方阿胶浆(山东东阿阿胶股份有限公司生产,批号050446);贞芪扶正颗粒(甘肃扶正药业科技股份有限公司生产,批号040803);复方皂矾丸(陕西郝其军制药有限责任公司生产,批号041014);当归补血口服液(郑州市协和制药厂生产,批号041122);环磷酰胺(上海华联制药有限公司生产,批号050101)。②选取清洁级昆明种小鼠150只,随机数字表法分为15组,10只/组:1～3组分别灌服参芪颗粒混悬液3,2,1g/kg;4～6组分别灌服复方阿胶浆30,20,10mL/kg;7～9组分别灌服贞芪扶正颗粒混悬液15,10,5g/kg;10～12组分别灌服复方皂矾丸混悬液2.4,1.6,0.8g/kg;第13组灌服当归补血口服液10g/kg;剩余2组为空白对照组和模型对照组,分别给于同体积生理盐水10g/kg。各组给药1次/d,连续给药10d。③除空白对照组外,其他各组从给药第1天开始建立气血双虚模型。每只鼠尾部放血0.25mL/10g,然后分别于第2,4,6,8天腹腔注射环磷酰胺80,40,40,40mg/kg。空白对照组同时间点仅腹腔注射等体积生理盐水。末次注射环磷酰胺后2h,眼眶取血,一部分用于血象测定,另一部分离心取血清,测定血细胞比容及血清中巨噬细胞集落刺激因子水平;解剖取胸腺和脾脏,检测胸腺皮质厚度、胸腺淋巴细胞数、脾小结大小、脾脏淋巴细胞数病理学指标的变化。结果:150只小鼠全部进入结果分析,放血和注射环磷酰胺并用可成功建立小鼠气血双虚模型。①与模型对照组比较,参芪颗粒3g/kg组、贞芪扶正颗粒10,5g/kg组、复方皂矾丸1.6gAIM： To select effective Chinese medicine for bone marrow suppression induced by anti-AIDs therapy, and analyze the effects of Shenqi granule, compound Ejiao syrup, Zhenqifuzheng granule and compound melanteritum pill on the hemogram and immunity of mice with deficiency in Qi and blood established by bloodletting and injection of cyclophosphamide. METHODS： The experiment was conducted in the Laboratory of Pharmacology, Henan College of Traditional Chinese Medicine between August and September 2005. ①Shenqi granule （manufactured by Jiangxi Shangao Pharmaceutical Co., Ltd with the batch number of 040702）; compound Ejiao syrup （manufactured by Shandong Dongejiao corporation with the batch number of 050446）; Zhenqifuzheng granule （manufactured by Gansu Fuzheng Pharmaceutical company with the batch number of 040803）; Compound melanteritum pill （manufactured by Shanxi Haoqijun Pharmaceutical Company with the batch number of 041014）; Danggui blood-enriching oral lipid （manufactured by Zhengzhou Xiehe Pharmaceutical Company with the batch number of 041122）; Cyclophosphamide （manufactured by Shanghai Hualian Pharmaceutical Company with the batch number of 050101 ）. ② 150 Kunming clean mice were randomly divided into 15 groups with 10 in each group： Mice in the first 3 groups were drenched with Shenqigranule suspension respectively at 3, 2 and 1 g/kg. Mice in the 4^th-6^th groups were drenched with compound Ejiao syrup respectively at 30, 20 and 10 mL/kg. Mice in the 7^th-9^th groups were drenched with Zhenqifuzheng granule suspension respectively at 15, 10 and 5 g/kg. Mice in the 10^th-12^th groups were drenched with compound melanteritum pill suspension respectively at 2.4, 1.6 and 0.8 g/kg. Mice in the 13^th group were drenched with Dangguiblood-enriching oral liquid at 10 g/kg, while mice in the other 2 groups were taken as the blank control group and model control group and administrated with normal saline at the same dose. Mice in all groups were administrated once a day for 10 conti

关 键 词：中成药 血细胞计数 免疫学 

分 类 号：R229[医药卫生—中医基础理论]