脑出血周围组织细胞凋亡与bcl-2及bax蛋白表达的关系  

Apoptosis and expressions of bcl-2 and bax protein in perihematomal brain regions of rats

作　　者：李红玲[1] 赵鹏飞[2] 袁正华[1] 李春岩[3] 

机构地区：[1]河北医科大学第二医院康复科 [2]河北省石家庄市中医院康复科 [3]河北医科大学第二医院神经内科,050000

出　　处：《中国康复医学杂志》2007年第3期222-224,I0001,共4页Chinese Journal of Rehabilitation Medicine

摘　　要：目的:探讨脑出血灶周围组织中bcl-2,bax蛋白表达和细胞凋亡的关系。方法:SD大鼠70只,随机分为两组(实验组和对照组)。实验组采用胶原酶诱导大脑尾状核脑出血模型,分别于术后第6h、12h、24h、48h、72h、7d、14d,7个时相点(每个时相点5只)处死大鼠,运用TUNEL法、免疫组化SP技术,检测血肿周围脑组织中细胞凋亡及bcl-2,bax蛋白表达。结果:实验组细胞凋亡、bcl-2,bax蛋白表达与对照组有非常显著性差异(P<0.01),高峰期分别为脑出血术后第6h、48h。bcl-2蛋白表达与凋亡阳性细胞数呈负相关(r=-0.7628,P<0.05),bax蛋白表达与凋亡阳性细胞呈正相关(r=0.8438,P<0.01)。结论:脑出血灶周围脑组织中存在细胞凋亡,bcl-2,bax蛋白对凋亡具有调控作用。Objective：To investigate the relations between the expressions of bcl-2 and bax protein and apoptosis in perihematomal brain regions of rats. Method： Seventy SD rats were individed randomly two groups,experimental group and control group. A model of ICH was establisbed by stereotactically injection of 0.SU bacterial collagenase VK into candate nucleus in the rats. Rats in two groups were executed after 6h,12h,24h,48h,72h,Td and 14d,respectively. TUNEL method was used to detect apoptosis,and SP method to detect expressions of bcl-2 and bax protein in the perihematomal brain tissues.Result：The apoptosis and expressions of bcl-2 and bax protein were significant different in rats of experimental group compared to the rats of control group. The peak value were at the 6th, 48th hour, respectively. The expression of bcl-2 protein was negatively correlated with the number of apoptotic cells （r=- 0.7628,P〈0.05）. The expression of hax protein was positively correlated with the number of apoptotic cells （r= 0.8438,P〈0.01）. Conclusion： Apoptosis may present and reflect brain damage after intracerebral hemorrhage. Gene bcl-2 and bax may play an important role during the period of apoptosis.

关 键 词：脑出血 细胞凋亡 BCL-2蛋白 bnx蛋白 大鼠 胶原酶 

分 类 号：R743.34[医药卫生—神经病学与精神病学]