4’-甲醚-黄芩素对绒毛膜癌JAR细胞内Ca^(2+)浓度的影响  

Effects of 4'-Methery-scutellarein on intracellular calcium concentration in human chorio-carcinoma JAR cell line

作　　者：杨最素[1] 罗莉[1] 朱利群[1] 仇黎丽[1] 徐昌芬[1] 

机构地区：[1]南京医科大学组织胚胎学教研室

出　　处：《南京医科大学学报（自然科学版）》2007年第3期228-231,F0003,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省自然科学基金资助项目(BK2003104)

摘　　要：目的:探讨4′-甲醚-黄芩素对人绒毛膜癌JAR细胞的增殖抑制作用及细胞内Ca2+浓度的影响。方法:应用MTT法、流式细胞术和激光共聚焦扫描显微镜技术,体外观察该化合物对JAR细胞的影响。结果:4′-甲醚-黄芩素对JAR细胞的生长具有抑制作用。随着药物浓度与作用时间的增加,Annexin Ⅴ-FITC+/PI-细胞、细胞内Ca2+浓度逐渐增加,与对照组相比差异有显著性。结论:4′-甲醚-黄芩素能抑制人绒毛膜癌JAR细胞的增殖,诱导凋亡,其机制与提高细胞内Ca2+浓度有关。Objective：To investigate the effects of 4＇-methery-scutellarein （4-MS） on proliferation and intracellular calcium concentration in human choriocareinoma JAR cell line. Methods：MTT assay, flow cytometry and laser scanning confocal microscopy were used to detect the effects of 4-MS on JAR cells. Results：4-MS inhibited the growth of JAR cells. Annexin V-FITC^＋/PI^- cells and intracellular calcium concentration were gradually increased with the increase of the dose and action time of 4-MS. There were significant differences compared with the control group. Conclusion ：4-MS can inhibit the proliferation and induce apoptosis of human choriocarcinoma JAR cell line. Its mechanism is related to the increase of intracellular calcium concentration.

关 键 词：4'-甲醚-黄芩素 JAR细胞 凋亡 细胞内CA^2+浓度 

分 类 号：R737.33[医药卫生—肿瘤] R961[医药卫生—临床医学]