不同化疗方案对裸鼠MCF-7乳腺癌移植瘤PCNA和Bcl-2表达的影响  

The effects of various chemotherapy regimens on the expression of PCNA and Bcl-2 in human breast cancer xenograft (MCF-7) transplanted in nude mice

作　　者：王玉栋[1] 刘巍[1] 纪芝民[1] 张志刚[1] 吕雅蕾[1] 王淑琴[1] 

机构地区：[1]河北医科大学第四医院肿瘤内科

出　　处：《临床肿瘤学杂志》2007年第3期173-176,共4页Chinese Clinical Oncology

摘　　要：目的:研究不同化疗方案对乳腺癌组织PCNA和Bcl-2的影响,探讨二者与化疗的关系及评价疗效的价值。方法:制备MCF-7乳腺癌荷瘤裸鼠模型,化疗后观察移植瘤病理组织学疗效,用免疫组化SP法显示乳腺癌组织PCNA和Bcl-2表达情况。结果:(1)各化疗组瘤组织PCNA表达显著低于对照组(P<0.05),且NP、TP和Xeloda组显著低于CMF、CAF组(P<0.05)。PCNA表达与病理疗效显著相关(P=0.001)。(2)CAF、NP、TP和Xeloda化疗组Bcl-2蛋白表达显著高于对照组(P<0.05),且TP组显著高于CMF、CAF组(P<0.05)。Bcl-2表达与病理疗效无显著相关性(P=0.093)。结论:化疗可降低乳腺癌组织PCNA表达,并增强Bcl-2的表达,且不同化疗方案对二者影响的差异有显著性。PCNA可作为评价乳腺癌化疗效果的参考指标,对选择化疗方案可能有指导意义。Objective：To investigate the effects of various chemotherapy regimens on the expression of PCNA and Bcl-2 of breast cancer. To assess relationships between chemotherapy and two markers, and to evaluate the value of them to predict the response of chemotherapy. Methods： Forty-eight nude mice models of human breast cancer xenograft （MCF-7） were established, and then were randomly divided into control and 5 chemotherapy groups （ each group, n = 8）. Among 5 chemotherapy groups, mice were treated intraperitoneally／oral by 5 chemotherapy regimens （CMF, CAF, NP, TP, Xeloda） respectively at two-thirds LD10 （dose lethal to 10% of the mice）. Control animals were administered intraperitoneally with normal saline. The pathological feature of transplanted tumor was studied by HE stain, and the expression of Bcl-2 and PCNA was studied by SP immunohistochemical method. Results： （ 1 ） The expression of PCNA in 5 chemotherapy group was significantly lower than that of control （ P 〈 0. 05 ）, and the expression of PCNA in NP, TP and Xeloda groups was significantly lower than that of CMF and CAF groups （P 〈 0. 05）. Moreover, the expression of PCNA was significantly correlated with pathological therapeutic response （ P = 0. 001 ）. （ 2 ） The expression of Bcl-2 in CAF, NP, TP, Xeloda groups was significantly higher than that of control （P 〈 0.05 ）. Moreover, the expression of Bcl-2 in TP group was significantly higher than that of CMF and CAF groups （P 〈 0. 05）. The expression of Bcl-2 was not significantly correlated with the pathological therapeutic response （ P = 0. 093 ）. Conclusion： Chemotherapy can increase the expression of PCNA, and decrease the expression of Bcl-2. Different chemotherapy regimens have different effects on PCNA and Bcl-2. PCNA can become a factor to evaluate the response to chemotherapy, and become possibly the prospective factor of chemoselect.

关 键 词：乳腺癌 MCF-7 化疗 PCNA Bcl-2 

分 类 号：R730.53[医药卫生—肿瘤] R737.9[医药卫生—临床医学]