过氧化物酶体增殖物激活受体激动剂治疗急性胰腺炎的实验研究  

Effect of peroxisome proliferator-activated receptor-γ (PPAR γ) ligand on acute pancreatitis in rats

作　　者：聂盛丹[1] 吕品[1] 苗雄鹰[2] 吴金术[1] 

机构地区：[1]湖南省人民医院,湖南长沙410005 [2]中南大学湘雅二医院,湖南长沙410005

出　　处：《中国现代医学杂志》2007年第5期539-542,共4页China Journal of Modern Medicine

摘　　要：目的探讨PPAR-γ激动剂罗格列酮对急性胰腺炎大鼠的保护作用及其机制。方法腹腔注射蛙皮素制成AP大鼠模型。64只健康SD大白鼠随机分为4组,假手术组,AP组,两个剂量的罗格列酮治疗组,分别于术后6和12h,取血检测血浆淀粉酶、TNF-α水平,术后6h取胰腺组织,观察病理损伤情况,并检测NF-кB活性。结果AP大鼠的淀粉酶、TNF-α水平、胰腺组织病理学评分及NF-кB活性较对照组明显增高,(P<0.05)。给予罗格列酮治疗后,淀粉酶、TNF-α水平、胰腺组织病理学评分和NF-кB活性显著降低。结论PPAR-γ激动剂罗格列酮可有效地减轻急性胰腺炎大鼠胰腺损伤,其机制与降低NF-кB活性及TNF-α水平有关。[Objective] To study the protective effect of Roglitazone, a specific peroxisome proliferator-activated receptor-γ （PPARγ） ligand on acute pancreatitis （AP） and explore its mechanism. [Methods] AP was induced by injection of Cerulein. The Sprague-Dawley rats were randomly divided into four groups （n =16 in each group）, ineluding sham operation group, rats were treated with sham operation and saline; rats in AP group were induced pancreatitis and treated with saline; two Roglitazone treated groups, rats were induced pancreatitis and treated with roghtzone 0.3 mg/kg, 3 mg/kg respectively. At 6 h and 12 h after induction of AP, blood sample was withdrown for the analysis of amylase activity and TNF-α level. At 6 h, rats were sacrificed , histologic injury and the activity of NF-kappaB in pancreatic tissues were examined. [Results] Compared with AP group, the level of TNF-α, pathological scores in pancreatic tissues, and the activity of NF-kappaB were significantly lower in two Roglitazone treated groups （P 〈0.05）. There was no obvious difference in amylase activity among the three groups. [Conclusion] Our findings demonstrate that Roglitazone can effectively attenuate the severity of AP in rats. This attenuation is associated with the decreased TNF-α levels, and is most likely through the inhibition of NF-kappaB activity.

关 键 词：急性胰腺炎重症 核因子-KB 肿瘤坏死因子-Α PPAR-Γ激动剂 罗格列酮 

分 类 号：R576.1[医药卫生—消化系统]