两株具有抗癌活性内生细菌的分离及分类  

Isolation and Classification of Two Anticancer Endophytic Bacterial Strains

作　　者：李洁[1] 陈华红[1] 赵国振[1] 熊智[2] 徐丽华[1] 

机构地区：[1]云南大学云南省微生物所微生物药物国家工程研究中心云南基地,云南昆明650091 [2]西南林学院资源学院,云南昆明650224

出　　处：《微生物学杂志》2007年第1期1-4,共4页Journal of Microbiology

基　　金：国家重大基础研究发展规划项目(973项目;2004CB719601);国家自然科学基金项目(30560001);云南省国际合作计划(2005GH21);云南省自然科学基金(2004C0002Q);新世纪优秀人才支持计划项目资助

摘　　要：从西双版纳植物样品爵床[Rostellularia procumbens(L.) Nees]中分离到2株具有较强抗癌活性的内生细菌YIM 56077和YIM 56081。通过对其进行表型特征、生长及生理生化特性、细胞化学组份以及系统进化分析,发现这2株菌与B.flexus IFO15715T的亲缘关系最近,但它们在生长、生理生化等特征上表现明显差异,是芽胞杆菌属的2个具有开发潜力的新菌株。Two endophytic strains YIM 56077 and YIM 56081 possessing great anticancer activity were isolated from plant sample of Rostellularia procumbens collected from Xishuangbana, Yunnan Province. Their morphology, physiological, biochemical characteristics, chemotaxonomy, and 16S rDNA sequence were analyzed. The 16S rDNA gene sequence of YIM 56077 and YIM 56081 exhibited maximum similarity with that of Bacillus flexus IFO15715^T, however, they were apparently different in growth and physiological and biochemical characteristics. They are two new strains potential of development in the genus of Bacillus.

关 键 词：内生菌 芽胞杆菌属 抗癌活性 系统发育 

分 类 号：Q939.92[生物学—微生物学]