人食管下括约肌M2和M3受体的基因表达  

Expression of muscarinic receptor subtypes M2 and M3 in lower esophageal sphincter.

作　　者：陈新[1] 刘俊峰[1] 王其彰[1] 李保庆[1] 王福顺[1] 王士杰[1] 

机构地区：[1]河北医科大学第四医院胸外科,石家庄050011

出　　处：《中国综合临床》2007年第4期363-364,共2页Clinical Medicine of China

摘　　要：目的 探讨人食管下括约肌的胆碱能受体基因表达和分布的情况，从分子水平揭示食管下括约肌的抗返流机制。方法 应用RT-PCR方法，研究钩状纤维和套索纤维中M2、M3的分布与表达。结果 钩状纤维、套索纤维M2和M3均高表达，M2的表达水平分别为0．411±0．023和0．409±0．044，M3的表达水平分别为0．141±0．016和0．145±0．023，而且M2比M3有较高的表达，两者差异有统计学意义（P〈0．01）．结论 在钧状纤维、套索纤维中．存在丰富的M2、M3,同时与M3相比，M2处于优势。Objective To explore the expression and distribution of M2 and M3 in the lower esophageal sphincter （ LES ） and to uncover the mechanism of anti-reflux in LES from molecular level. Methods RT-PCR was used to examine the expression of M2 and M3 in clasp fibers and sling fibers. Results The expression of M2 of clasp fibers and sling fibers were 0.411±0.023 and 0. 409 ±0.044 respectively. The expression of M3 of clasp fibers and sling fibers were 0. 141 ±0. 016 and 0. 145 ±0. 023. The expression level of M2 was significantly higher than that of M3 in LES （ P 〈 0.01 ）. Conclusion The expression level of M2 and M3 is high in clasp fibers and sling fibers. M2 plays a more important role than M3 in physiological function.

关 键 词：食管下括约肌 食管胃黏膜线 钩状纤维 套索纤维 逆转录多聚酶链反应 

分 类 号：R57[医药卫生—消化系统]