依达拉奉对少突胶质细胞缺血性反应的保护作用研究  

Effects of edaravone on ischemic oligodendrocyte in rats

作　　者：陈应柱 田野[2] 包仕尧[2] 吴冠会[2] 许俊 袁成林 

机构地区：[1]扬州大学临床医学院神经内科,江苏扬州225001 [2]苏州大学附属第二医院神经内科,江苏苏州215006

出　　处：《中风与神经疾病杂志》2007年第1期41-43,I0001,共4页Journal of Apoplexy and Nervous Diseases

基　　金：江苏省卫生厅重大科研课题资助项目(K200406);苏州大学医学发展基金(EE123504)

摘　　要：目的观察依达拉奉对脑缺血大鼠脑内少突胶质细胞(OLG)的影响。方法将雄性SD大鼠50只随机分为假手术组、对照组、依达拉奉组。采用四血管闭塞全脑缺血模型,制模后依达拉奉组大鼠分别按剂量0．3、1．0和3．0mg/kg依达拉奉腹腔注射每日1次,连续7d;构建缺血性脑损伤大鼠脑组织芯片,用免疫组化法检测皮质A2B5、少突胶质细胞表面标志物4(O4)、2’,3’-环磷核苷酸水解酶(CNPase)蛋白表达变化。结果与假手术组比较,对照组皮质A285阳性细胞数增多,O4、CNPase阳性细胞数减少(均P＜0．05);与对照组比较,依达拉奉干预后A2B5阳性细胞数有不同程度减少,O4、CNPase阳性细胞数有不同程度增加(1mg/kg P＜0．05,3mg/kg P＜0．01)。结论一定剂量的依达拉奉对OLG缺血性反应有保护作用,呈现剂量依赖性。Objective To investigate the effects of edaravone on the ischemic reaction of oligodendroeyte in rats. Methods 50 male Sprague-Dawley rats were randomly divided into sham-operated group,control group and edaravone group. The model of whole-brain ischemia was established by exposuring the brains of rats to four-vessel occlusion（4-VO）. The rats were injected intraperitoneally with edaravone of 0. 3mg/kg,1mg/kg,3mg/kg once a day for seven days after 4-VO. Tissue microarry of ischemic brain injury in rats was constructed. The expression of A2B5,oligodendrocyte marker 4 （O4） and 2＇, 3＇ -cyclic nucleotide 3＇ -phosphodiesterase （CNPase） in t he cortex was examined by tissue microarray technology and immunohistochemistry. The positive cells were counted. Results Compared with sham operated group,the number of A2BS-positive cells increased and the number of O4,CNPase-positive cells decreased significantly in the control group（P〈0. 05）. Compared with model group ,A2B5-positive cells decreased significantly after edaravone treatment,while O4-positive cells and CNPase-positive cells increased signifieantly（1mg/kg P〈0. 05,3mg/kgP〈0. 01）. Conclusion Edaravone plays a protective role in oligodendrocyte ischemic reaction at a dose-dependent manner.

关 键 词：少突胶质细胞 依达拉奉 全脑缺血 组织芯片 

分 类 号：R743.3[医药卫生—神经病学与精神病学]