丹参酮ⅡA抑制Hela细胞生长及诱导凋亡的体外实验研究  

Effects of TanshinonⅡA on proliferation and apoptosis of hHuman cervical carcinoma Hela cells in vitro

作　　者：王冬[1] 田亚平[1] 姜英雁[1] 任宝红[1] 杨宁江[1] 方青[1] 李英女[1] 

机构地区：[1]吉林医药学院附属医院,吉林吉林132013

出　　处：《中国现代医学杂志》2007年第6期676-678,682,共4页China Journal of Modern Medicine

摘　　要：目的探讨丹参酮ⅡA对宫颈癌细胞生长抑制及诱导细胞凋亡作用的研究。方法采用体外宫颈癌细胞培养方法取0～8.0μg/mL浓度的丹参酮ⅡA作用Hela细胞,72h后,观察细胞生长抑制情况,电镜观察细胞凋亡的形态学变化。流式细胞仪检测不同浓度丹参酮ⅡA对细胞凋亡的影响。结果丹参酮ⅡA作用于Hela细胞后,对该细胞有明显的抑制作用,72h后0.5、1.0、2.0、4.0和8.0μg/mL不同浓度的丹参酮ⅡA对细胞生长的抑制率分别为17.23%、24.27%、31.75%、39.37%和55.45%。与对照组比较差异有显著性。电镜显示:核皱缩、核碎裂、形成凋亡小体。流式细胞仪测定72h后不同丹参酮ⅡA浓度与细胞凋亡率分别为0.5、1.0、2.0、4.0和8.0μg/mL;12.37%、25.81%、27.98%、34.13%和45.84%。与对照组比较,0μg/mL;2.09%差异均有显著性。结论丹参酮ⅡA对宫颈癌细胞生长有明显的抑制作用并诱导宫颈癌细胞凋亡。[Objective] To investigate the regulatory effect of Tanshinon ⅡA on proliferation and apoptosis of human cervical carcinoma Hela cells in vitro. [Methods] Human cervical carcinoma cell line Hela was cultured in vitro. Hela cell were treated with 0.5-8.0 p.g/L Tanshinon ⅡA for 24-72 h and the growth inhibition rate were measured with MTT method. Cell apoptosis was inspected by electron microscopy, apoptotic rate was quantified flow cytometry （FCM）. [Results] After treatment with 0.5-8.0 p.g/L Tanshinon ⅡA for 72 h the proliferation of Hela cells was significantly inhibited, the growth inhibition rate was 17.23%, 24.27%, 31.75%, 39.37%, 55.45%, respectively. Cell apoptosis occurred characterized by cell shrinkage, nuclear chromatin condensation and fragmentation. Formation of membrane blebs and apoptosis bodies as observed under fluorescence microscope and transmission electron microscope. The apoptosis rates quantified tanshinon ⅡA 0.5～8.0 μg/L for 72 h by flow cytometry （FCM） were 12.37%, 25.81%, 27.98%, 34.13%, 45.84%. [Cunelusion] Tanshinon ⅡA could significantly inhibit the growth of Hela cell. Tanshinon ⅡA can induce the apoptosis of human cervical carcinoma Hela cells in vitro, which may be related to the mechanism of growth inhibition of human cervical carcinoma Hela cells line.

关 键 词：丹参酮ⅡA 宫颈肿瘤 细胞培养 细胞凋亡 

分 类 号：R737.33[医药卫生—肿瘤] Q255[医药卫生—临床医学]