阿司匹林、塞莱昔布对胃癌BGC-823细胞株增殖的影响及机制探讨  

Effect and mechanism of asprin and celecoxib on human gastric cell lines BGC-823 proliferation

作　　者：陈怡[1] 宋伟庆[2] 韦金英[1] 刘玉[1] 王烨[1] 韩彩丽[1] 

机构地区：[1]河北医科大学,河北石家庄050017 [2]河北医科大学附属第二医院

出　　处：《山东医药》2007年第9期10-12,共3页Shandong Medical Journal

基　　金：河北省科技厅资助项目(303485)。

摘　　要：目的观察阿司匹林、塞莱昔布对胃癌BGC-823细胞株增殖的影响,并探讨其机制。方法采用M TT法检测阿司匹林和塞莱昔布对胃癌BGC-823细胞株增殖的影响;采用流式细胞术检测二者对BGC-823细胞株凋亡和细胞周期分布的影响;采用间接免疫荧光法检测二者对BGC-823细胞株环氧化酶-2蛋白表达的影响。结果阿司匹林、塞莱昔布以剂量依赖方式抑制BGC-823细胞增殖,促进细胞凋亡和细胞中环氧化酶-2蛋白的表达,并使细胞处于G2、M、S期。结论阿司匹林和塞莱昔布可通过诱导胃癌BGC-823细胞株和影响其细胞周期分布而抑制细胞增殖,其机制可能与抑制环氧化酶-2蛋白表达有关。[Objective] To observe the effects of aspirin and celecoxib on human gastric cell lines BGC-823, and explore the mechanism. [Methods] The effects of NSAIDs on BGC-823 cells＇ growth were determined with MTT assay and flow cytometry（FCM）. Apoptosis rate ,expression of COX-2 protein and cell cycle were detected with indirect immunofluorescence. [Results] NSAIDs inhibited the growth of BGC-823 cell line. They could promote cell apoptosis and the expression of COX-2,and make the cell lines stay at G2M period and S period. [Conclusion] Aspirin and celecoxib inhibit the growth of human BGC-823 cell line by inducing apoptosis and cell cycle arrest. The mechanism may beconcerned with the inhibition of COX-2 expression.

关 键 词：阿司匹林 塞莱昔布 胃肿瘤 细胞凋亡 环氧化酶-2 

分 类 号：R361[医药卫生—病理学] R735.2[医药卫生—基础医学]