温度、铜离子对鲤鱼血清HSP70合成的影响  

Effects of temperature and copper on HSP70 expression in carp serum

作　　者：盛连喜[1] 于文广[1] 徐镜波[1] 郑永晨[2] 王云彪[1] 

机构地区：[1]东北师范大学城市与环境科学学院,吉林长春130024 [2]吉林大学第二医院,吉林长春130022

出　　处：《东北师大学报（自然科学版）》2007年第1期108-113,共6页Journal of Northeast Normal University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(30370262);高等学校博士学科点专项科研基金资助项目(20040200002);国家重点基础研究发展计划(973)项目(2004CB418505)

摘　　要：为了研究温度、铜离子对鲤鱼血清热应激蛋白(HSP70)合成的影响,探讨HSP70的表达机制,以健康1龄鲤鱼(Cyprinus carpio)为受试生物,10℃下驯养,经过24 h不同温度(10℃,20℃,24℃,28℃)和系列铜离子浓度(0,10,50,100,300,500μg/L)暴露,采用双抗体夹心酶联免疫吸附剂检测法(enzyme-linked immunosorbnent assay,ELISA)检测鲤鱼血清HSP70合成水平,原子吸收光谱法(atomic absorption spectroscopy,AAS)检测血清铜离子含量.结果表明:20℃,24℃热冲击组鲤鱼血清HSP70合成水平高于10℃组,与10℃组相比,差异极其显著(P<0.01);铜离子能够诱导鲤鱼血清HSP70表达,呈现明显的剂量效应关系,热冲击与铜离子具有联合效应.To determine the effects of temperature and copper on carp （ Cyprinus carpio） serum HSP70, after acclimation at 10℃, healthy carps were exposed to combinations of three temperature levels （ 10℃, 20℃ ,24℃ ） and six copper concentrations（0,10,50,100,300,500 μg/L） for 24 h. HSP70 expression relative to the controls was analyzed by sandwish ELISA （enzyme - linked immunosorbnent assay, ELISA） ; Copper concentrations in fish serum were measured by atomic absorption spectroscopy （AAS）. The results showed that the HSP70 expression in carp serum under 20℃, 24℃ are both much higher than that under 10℃ （P〈 0.01）. Observed the copper could induce HSP70 expression in carp serum with significant loga- rithmic dose-effect relationship. There was strong combined effect of temperature and copper on HSP70 expression in this study.

关 键 词：鲤鱼血清 HSP70 ELISA 温度 铜离子 

分 类 号：X171.5[环境科学与工程—环境科学]