磺胺喹噁啉酶联免疫分析方法的建立  

Establishment of Enzyme-Linked Immunosorbent Assay for Sulfaquinoxaline

作　　者：刘超[1] 刘一兵[1] 韩世泉[1] 许文革[1] 张莉玲[1] 

机构地区：[1]中国原子能科学研究院同位素研究所,北京102413

出　　处：《原子能科学技术》2007年第2期142-147,共6页Atomic Energy Science and Technology

摘　　要：利用抗磺胺喹啞啉（SQ）多抗建立酶联免疫分析方法，以测定SQ在动物源食品中的残留。方法学鉴定结果表明：灵敏度为o．2ng／mL，批内、批间变异分别为7．8％～10．0％、10．5％～12．0％；牛奶、蜂蜜、鸡肉、鸡蛋样品的添加回收率分别在93．1％～107．0％、69．0％～104．0％、40．0％～73．6%、50．0％～84．0％之间。牛奶稀释实验表明，测定值与稀释度呈线性相关，相关系数R=0．997。An indirect competitive enzyme-linked immunoassay（ELISA） was developed using polyclonal antibody to measure the residues of sulfaquinoxaline（SQ） in food. The range of standard curve is 0.3-100 ng/mL. The sensitivity of the assay is 0.2 ng/mL. The intra-assay and inter-assay CVs are 7.8%-10.0%, 10.5%-12.0%, respectively. The recovery of SQ in milk, honey, chicken and eggs is 93. 1%-107. 0%, 69. 0%- 104.0%, 40. 0%-73. 6%, 50. 0%-84. 0%, respectively. The correlation coefficient between measured and expected value is 0. 997 after serial dilutions of the samples with high concentration of SQ.

关 键 词：磺胺喹啞啉 酶联免疫分析 兽药残留 

分 类 号：S859.7[农业科学—临床兽医学] S852.43[农业科学—兽医学]