基因工程菌在厌氧膜生物反应器中对偶氮染料废水的脱色  

Decoloration of azo dye wastewater by an anaerobic membrane bioreactor using gene engineering strain Escherichia coli JM109 (pGEX-AZR)

作　　者：丁华[1] 金若菲[1] 周集体[1] 

机构地区：[1]大连理工大学环境与生命学院,大连116024

出　　处：《环境工程学报》2007年第3期25-29,共5页Chinese Journal of Environmental Engineering

基　　金：辽宁省自然基金资助项目(20032122)

摘　　要：印染和染料废水色度大,COD高,可生化性差。采用基因工程菌Escherichia coli JM109(pGEX-AZR)在厌氧膜生物反应器中,对模拟偶氮染料废水进行脱色研究。结果表明,系统对酸性红B有很好的脱色能力,且启动期短,脱色效率高,脱色率稳定在95%以上,对COD的去除率能达到68%。系统运行过程中,反应器内生物量稳定在0.4 g/L。EPS与生物量变化趋势一致,先升高后降低;pH值在运行初期由6.5降至6.2,后维持在6.6左右。系统运行27 d后进行第一次膜清洗,膜通量恢复为初始值的92%,系统运行周期约为26 d。Among industrial wastewaters, dye wastewater from the textile or dyestuff industries is one of the most difficult to treat. In this study an anaerobic membrane bioreactor （MBR） using gene engineering strain JM109 （pGEX-AZR） was used for the decoloration of azo dye solutions. The results from the study showed that the bioreactor with JM109 （pGEX-AZR） had a high capacity for decoloration of acid red B. The decoloration efficiency and COD degradation rate exceeded 95% and reached 68% , respectively. As in the continuous operation, the biomass increased from 0. 58 g/L to 0. 82 g/L, and then remained stable at 0. 4 g/L. Influenced by the bacteria activity, EPS changed with the biomass. The pH value fell from 6.5 to 6. 2 in the beginning, and remained stable at 6.6. The membrane was washed for the first time after 27 days, and the water membrane flux could recover 92%. The period of the bioreactor processing was 26 days.

关 键 词：基因工程菌 膜生物反应器 偶氮染料 脱色 

分 类 号：X172[环境科学与工程—环境科学]