导入针对survivin基因的siRNA对大肠癌Lovo细胞凋亡和增殖的影响  

Apoptosis and cell proliferation of colon carcinoma Lovo cells after transient transfection siRNA targeting survivin gene

作　　者：熊英[1] 郭文[1] 李霆[1] 

机构地区：[1]南方医科大学南方医院消化科,广州市510515

出　　处：《实用诊断与治疗杂志》2007年第4期259-261,共3页Journal of Practical Diagnosis and Therapy

基　　金：2006年广东省社会发展领域科技项目课题;编号:63005

摘　　要：目的:向大肠癌离体Lovo细胞中导入针对survivin基因的siRNA,并研究其对Lovo细胞凋亡的影响。方法:设计2条特异性针对survivin基因的siRNA,并体外转录法合成,利用脂质体导入Lovo细胞并检测转染后细胞的凋亡和增殖情况。结果:RT-PCR检测survivin mRNA表达水平发现两RNA干扰组细胞内survivin mRNA表达量明显低于正常Lovo细胞;细胞凋亡检测结果发现两干扰组细胞凋亡率分别为21．5％和26．28％明显高于正常Lovo细胞;细胞增殖结果显示:两干扰组细胞增殖能力比正常Lovo细胞减弱。结论:针对survivin基因的siRNA能有效降低目的基因的mRNA表达,抑制Lovo细胞生长,细胞凋亡率明显增高。为大肠癌基因治疗的可行性提供了有力的证据。Objective To introduce survivin siRNA to colon carcinoma Lovo cells and study the influence on apoptosis and cell proliferation. Methods Two pairs of siRNAs specific targeting survivin gene were designed,synthesized by vitro transcription,and transfected into Lovo cells by lipofectamine 2000. The cell apoptosis and cell proliferation were detected. Results Survivin mRNA expression level was detected with RT-PCR. Two survivin-targeted siRNA groups＇ survivin mRNA expression levels were both lower than normal Lovo＇s. The cell apoptosis of two survivin-targeted siRNA groups was 21.5% and 26.28% ,both higher than normal Lovo＇s. The cell proliferation of two survivin-targeted siRNA groups was both lower than normal Lovo＇s. Conclusion The 2 siRNA targeting survivin gene can suppress the expression of survivin mRNA, inhibit cell growth,and make the apoptosis higher. It provides a powerful evidence of carcinoma of colon gene therapy.

关 键 词：suvivin 基因治疗 RNAI 细胞凋亡 

分 类 号：R735.3[医药卫生—肿瘤]