Expression and Purification of Active Recombinant Human Nerve Growth Factor from Escherichia coli  

作　　者：HE Ling-bing WANG Yan WANG Ge CHEN Chao CAO Shu-gui 

机构地区：[1]College of Life Science ＆ Biotechnology, Shanghai Jiaotong University, Shanghai 200240, P. R. China [2]Shenzhen Neptunus Interlong Bio-tech Holdings Co. , Ltd. , Shenzhen 518054, P. R. China [3]Key Laboratory for Molecular Enzymology ＆ Engineering of Ministry of Education, Jilin University, Changchun 130012, P. R. China [4]Shandong Younglong Biotechnology Inc. , Jinan 250101, P. R. China

出　　处：《Chemical Research in Chinese Universities》2007年第2期237-240,共4页高等学校化学研究（英文版）

基　　金：the National Natural Science Foundation of China(Nos 20432010, 30570405 and 20672045)

摘　　要：Introduction Nerve growth factor （NGF） was first discovered and purified by Rita Levi-Montalcini and Stanley Cohen in the 1950s. It represents the first cellular growth factor ever discovered and involved in the gl-owth ,survival, and differentiation of specific nerve cell populations. Although animal tests and trials indicate that rhNGF could be ment for diabetic and HIV-related phase- Ⅱ clinical an effective treatneuropathies , a large-scale phase-Ⅲ clinical trial has failed to give similar result. NGF isolated from the mouse submaxillary gland has been widely used clinically in China for the treatment of peripheral neuropathy,Introduction Nerve growth factor （NGF） was first discovered and purified by Rita Levi-Montalcini and Stanley Cohen in the 1950s. It represents the first cellular growth factor ever discovered and involved in the gl-owth ,survival, and differentiation of specific nerve cell populations. Although animal tests and trials indicate that rhNGF could be ment for diabetic and HIV-related phase- Ⅱ clinical an effective treatneuropathies , a large-scale phase-Ⅲ clinical trial has failed to give similar result. NGF isolated from the mouse submaxillary gland has been widely used clinically in China for the treatment of peripheral neuropathy,

关 键 词：Nerve growth factor Fusion protein PURIFICATION Biological activity 

分 类 号：R394.6[医药卫生—医学遗传学]