用大孔吸附树脂分离链格孢菌毒素  

Isolation of Alternaria alternata toxin using macroporous resins

作　　者：周兵[1] 安传福[1] 董云发[1] 强胜[1] 

机构地区：[1]南京农业大学杂草研究室,江苏南京210095

出　　处：《浙江林学院学报》2007年第2期198-202,共5页Journal of Zhejiang Forestry College

基　　金：国家高技术研究发展计划(863计划)项目(2001AA246012);江苏省科学技术攻关项目(BE2005349);江苏省自然科学基金资助项目(BK2005420);教育部博士点研究基金资助项目(20050307002)

摘　　要：研究了大孔吸附树脂DA201，D101，HZ-803，1300及活性炭对链格孢菌Alternaria alternata毒素的吸附性能。结果表明，DA201对链格孢菌毒素吸附解吸效果最佳，吸附率最高，为72．88％，吸附后滤出液毒性最弱，解吸滤液毒性最强，适合用于链格孢菌毒素的分离纯化富集。并以DA201为吸附材料，对无菌滤液的上样量、pH值和洗脱液乙醇的体积分数等条件进行了优化，得到DA201对毒素吸附和解吸附的最优条件是：无菌滤液的上样量体积为树脂体积的60～70倍，pH4．12～4．62，洗脱液为体积分数为90％的乙醇。To obtain right separation methods of Alternaria alternata toxin, absorbility of macroporous resins; DA201, D101, HZ-803, 1300 and activated carbon were compared , and the filtrate load, pH, and concentration of ethanol that influenced adsorption and desorption were designed. Results showed that DA201 had the greatest absorbility with best absorption and desorption in culture condition, a filtrate volume load of 60 to 70 times than resin volume, a pH of 4.12 - 4.62, and a 90% ethanol eluting solvent. Thus, DA201 was suitable for purification and enrichment of the Alternaria alternata toxin. [ Ch, 1 fig. 3 tab, 12 ref. ]

关 键 词：植物保护学 链格孢菌毒素 大孔吸附树脂 活性炭 吸附 解吸 

分 类 号：S451.1[农业科学—植物保护]