过氧化氢摄入对小鼠肾脏显微结构的影响  

Effect of Hydrogen Peroxide on Microstructure of Mice Kidney

作　　者：马全祥[1] 毛泽善[2] 袁向山[1] 韩金珠[1] 杨廷桐[2] 

机构地区：[1]新乡医学院组织学与胚胎学教研室,河南新乡453003 [2]新乡医学院形态学实验室,河南新乡453003

出　　处：《实用儿科临床杂志》2007年第5期377-378,共2页Journal of Applied Clinical Pediatrics

基　　金：河南省教育厅自然科学研究计划项目资助(2007310009);新乡医学院科研项目资助(2005YJ07)

摘　　要：目的研究过氧化氢摄入对小鼠肾显微结构的影响,探讨过氧化氢对小鼠肾的毒性作用。方法健康雄性昆明属小鼠30只,随机分为对照组和实验组。对照组小鼠喂食自来水,实验组小鼠代以0.3g/L和3g/L过氧化氢溶液喂食。10d后处死小鼠,取出肾,常规石蜡制片染色,光镜观察。结果实验组小鼠肾组织中,近曲小管细胞的胞质随双氧水剂量增加呈水样变性、空泡化。结论过氧化氢对小鼠肾脏有毒性作用。Objective To study the effect of hydrogen peroxide on microstructure of mice kidney and discuss the toxic effect on mice kidney. Methods Thirty healthy male mice of Kunming Genus were divided into 3 groups at random ： control group and two experimental groups. Running water was fed to control group for 10 days while 0.3,3 g/L hydrogen peroxide running water readily prepared was fed to the experimental groups for 10 days. On the 10^th day,the kidneys were taken out,and fixed in the fixation solutions,conventionally produced and stained. Finally,they were studied under the optical microscope. Results Experimental groups：in the kidney tissue cytoplasm of proximal convoluted tubule showed hydropie degeneration and vacuolation which depend on dose of hydrogen peroxide. Conclusion Toxic effect on mice kidney can be caused by hydrogen peroxide.

关 键 词：过氧化氢 肾 显微结构 小鼠 

分 类 号：R361[医药卫生—病理学]