肉芝软珊瑚Sarcophyton crassocaule中新的多羟基甾醇的分离  

Isolation of New Polyhydroxylated Sterol from Soft Coral Sarcophyton crassocaule Mosre

作　　者：张翠仙[1,2] 阎素君[1] 张广文[3] 苏镜娱[1] 曾陇梅[1] 

机构地区：[1]中山大学化学与化工学院 [2]广州中医药大学中药学院,广州510405 [3]暨南大学食品科学与工程系

出　　处：《高等学校化学学报》2007年第4期686-688,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:29932030);国家'八六三'计划项目(批准号:2001AA620403)资助.

摘　　要：In our continuing search of discovering bioactive substances from marine organisms, a sample of soft coral Sarcophyton crassocaule Mosre collected off the Sanya bay, China, was investigated. Two polyhydroxylated sterols 1 and 2 were isolated from the 95% ethanol extract of the sample by chromatography over silica gel. Compound 1, named sarcrasterol, was a new compound, its structure was established as(22R*,24ξ)-methyl-cholest-5-en-3β,22α,25,28-tetraol by spectroscopic methods. Compound 2, a known compound, was first obtained by Kobayashi, however, the relative configuration of C24 has not been determined yet. This paper reports the stereochemistry of compound 2 established as (24S*)-methyl cholest-3β,5β,6α,25-tetraol by means of X-ray single crystal diffraction analysis. Bioassay results show that compound 2 has a moderate activity against human tumor cell lines MGC-803 with an IC50 value of 23.0 μg/mL.In our continuing search of discovering bioactive substances from marine organisms, a sample of soft coral Sarcophyton crassocaule Mosre collected off the Sanya bay, China, was investigated. Two polyhydroxylated sterols 1 and 2 were isolated from the 95% ethanol extract of the sample by chromatography over silica gel. Compound 1, named sarcrasterol, was a new compound, its structure was established as （ 22R ^＊, 24ξ） -methyl-cholest-5-en-3β,22α, 25,28-tetraol by spectroscopic methods. Compound 2, a known compound, was first obtained by Kobayashi, however, the relative configuration of C24 has not been determined yet. This paper reports the stereochemistry of compound 2 established as （24S^＊）-methvl cholest-3β, 5β,6α,25-tetraol by means of X-ray single crystal diffraction analysis. Bioassay results show that compound 2 has a moderate activity against human tumor cell lines MGC-803 with an IC50 value of 23.0 ug/mL.

关 键 词：肉芝软珊瑚 次生代谢产物 多羟基甾醇 细胞毒性 

分 类 号：O629.61[理学—有机化学]