原发性肝癌中HIF-1α、P-gp的表达及相关性的研究  

Expression of HIF-1α and P-gp and their correlation in patients with hepatocellular carcinoma

作　　者：喻芳[1] 周庚寅[1] 张翠娟[1] 高鹏[1] 马超[1] 李红[1] 

机构地区：[1]山东大学齐鲁医院病理科,山东济南250012

出　　处：《山东大学学报（医学版）》2007年第3期246-249,共4页Journal of Shandong University:Health Sciences

基　　金：教育部博士点专项科研基金资助项目(20020422042)

摘　　要：目的:观察HIF-1α和P-gp在原发性肝癌(HCC)中的表达,探讨两者相互作用的关系。方法:采用免疫组织化学法检测36例HCC及20例非肿瘤肝组织中P-gp和HIF-1α的表达,对两者均表达阳性的病例,进行免疫组织化学双标记染色。结果:36例HCC中HIF-1α和P-gp高表达,阳性率分别为58.33%和77.78%,明显高于非肿瘤肝组织(P<0.001)。两者表达呈正相关(r=0.388,P<0.05)。18例两者均表达阳性的病例中,免疫双标共表达率为61.1%。结论:HCC中HIF-1α和P-gp的表达密切相关,HIF-1α可通过上调P-gp的表达实现对化疗药物的耐受,HIF-1α有望成为评价及逆转肿瘤多药耐药的新指标。Objective： To study the expression of hypoxia induced factor-1α （HIF-1α） and P-gp and their correlation in patients with hepatocellular carcinoma （HCC）. Methods： Immunohistochemistry was used to detect the expression of HIF-1α and P-gp in thirty-two HCC samples and twenty noncancerous liver samples. If the expressions of both HIF-1α and P-gp were positive in the same case, another slide was used to detect the co-expression of the two. Results： The positive expressions of HIF-1α （58.33%） and P-gp （77.78%） in HCC were significantly higher than those in the non-cancerous group （P 〈 0.001 ）. The expression of HIF-1α was positively correlated with the expression of P-gp （r = 0.388, P 〈0.05）. The co-expression rate was 61.1%. Conclusion： The expression of HIF-1α and P-gp has a close correlation in HCC, and HIF-1α may induce multi-drug resistance by up-regulating the expression of P-gp, and it may become a novel marker to evaluate and reverse multi-drug resistance.

关 键 词：肝肿瘤 P糖蛋白 缺氧诱导因子1Α 

分 类 号：R735.7[医药卫生—肿瘤]