D2-40标记的甲状腺乳头状癌中微淋巴管密度与淋巴结转移之间的关系  

D2-40 positive micro-lymphatic vessel density in thyroid papillary carcinoma correlate with lymphatic metastasis

作　　者：刘红[1] 张建平[1] 赵著梅[1] 王妍[1] 刘文君[1] 张泽立[1] 李红[1] 

机构地区：[1]山东大学医学院病理学教研室,山东济南250012

出　　处：《山东大学学报（医学版）》2007年第3期253-256,261,共5页Journal of Shandong University:Health Sciences

摘　　要：目的:应用新型淋巴内皮标记物D2-40分析甲状腺乳头状癌及周围组织微淋巴管密度(MLVD)与淋巴结转移之间的关系。方法:应用免疫组织化学Envison法检测46例甲状腺乳头状癌和20例结节性甲状腺肿中D2-40和CD34的表达,分别计数MLVD和微血管密度(MVD),分析其与甲状腺乳头状癌淋巴结转移之间的关系,同时与病变中MVD行对比观察。结果:91.3%甲状腺乳头状癌乳头轴心中的间质中无淋巴管。微淋巴管主要位于甲状腺乳头状癌周围结缔组织及被膜中。甲状腺乳头状癌周围结缔组织及被膜中的MLVD和MVD与淋巴结转移有密切关系,MLVD和MVD越高淋巴结转移率越高。同时甲状腺乳头淋巴管内的癌栓与淋巴结转移也显著相关(P<0.001)。结论:甲状腺乳头状癌的转移过程与微淋巴管及微血管的形成有密切关系。干预肿瘤转移抑制血管生成的同时也需要抑制淋巴管生成。Objective： Specimens of papillary thyroid carcinoma and nodular goiter have been immunohistochemically studied by using a novel monoclonal antibody D2-40 which is specific against lymph epithelial ceils in the detection of the newly formed micro-lymphatic vessel density（MLVD） in lesions in order to seek clues of papillary thyroid carcinoma and its well known ability to involve regional lymph nodes. Micro-vascular density（ MVD） as the future of tumor angiogenesis was immunochemically compared by CD34. Methods： MLVD and MVD were determined by conventional HE staining and Envison immunohistochemistry using D2-40 and CD34 monoclonal antibodies in 46 specimens of papillary thyroid carcinoma and 20 of nodular goiter. Results： The micro-lymphatic vessel of papillary thyroid carcinoma in the peripheral zone of stroma appeared to be associated with the spread of tumors to regional lymph nodes. Conclusion： The metastasis process of thyroid papillary carcinoma is associated with the formation of the micro-lymphatic vessel and micrangium. The antimetastatic potential for targeting these lymphatics may be of potential therapeutic benefit in the future.

关 键 词：甲状腺肿瘤 抗原 淋巴结转移 免疫组织化学 

分 类 号：R736.1[医药卫生—肿瘤]