血管紧张素Ⅱ含量及其Ⅰ型受体基因多态性与乙肝肝硬化的关系  

Correlation of Angiotensin Ⅱ and its type Ⅰ receptor gene polymorphisms with HBV-induced liver cirrhosis

作　　者：栗华[1] 赵幼安[2] 吕卉[3] 吴洪磊[4] 王洪波[4] 

机构地区：[1]福建医科大学附属厦门第一医院消化科,福建厦门361003 [2]山东大学齐鲁医院消化科,山东济南250012 [3]济南市传染病医院2科,山东济南250021 [4]山东大学第二医院消化科,山东济南250033

出　　处：《山东大学学报（医学版）》2007年第3期269-273,共5页Journal of Shandong University:Health Sciences

摘　　要：目的:探讨血管紧张素Ⅱ(AngⅡ)含量及Ⅰ型血管紧张素Ⅱ受体(AT1R)基因多态性与乙肝肝硬化的关系。方法:对102例乙型肝炎肝硬化患者和106例健康献血者,分别应用PCR-RFLP检测AT1R1166A/C基因多态性,并用放射免疫法检测血浆AngⅡ含量。结果:肝硬化组血浆AngⅡ含量明显高于对照组(P<0.05),肝硬化Child-Pugh C级、B级组的血浆AngⅡ含量明显高于A级组(P<0 05),而C级组与B级组的血浆AngⅡ含量无明显差异(P>0.05);肝硬化伴有腹水组和重度食管静脉曲张组血浆AngⅡ含量也明显高于无腹水组和轻度食管静脉曲张组,差异有统计学意义(P<0.05)。AT1R 1166A/C基因型频率和等位基因频率在肝硬化和对照组间差异均无统计学意义(P>0.05)。结论:肝硬化患者血浆AngⅡ含量明显升高,并与肝硬化严重程度及门脉高压症的发生密切相关,AT1R 1166A/C基因多态性可能与乙肝肝硬化遗传易感性无关。Objective： To investigate the correlation of polymorphisms of 1166 A/C of Angiotensin Ⅱ type Ⅰ receptor gene （ AT1R 1166A/C） and angiotensin Ⅱ with hereditary susceptibility and clinical phenotype of HBV-induced liver cirrhosis. Methods： Peripheral blood samples were collected from 102 patients with HBV-induced liver cirrhosis and 106 healthy donors. Polymorphisms of the 1166A/C of AT1R gene were determined by PCR-RFLP, and plasma concentrations of Angioteusin Ⅱ were measured by RIA. Results： Plasma concentrations of angioteusin Ⅱ of the liver cirrhosis group were significantly higher than those of the control group （ P 〈 0.05）. Plasma concentrations of angiotensin Ⅱ were also significantly higher in patients with the Child-Pugh C and B grades than those in patients with the Child-Pugh A grade （P 〈 0.01 and P 〈 0.05, respectively）. They were also higher in patients with severe esophageal varices and an ascites than those with light esophageal varices and without ascites （ P 〈 0.05）. There was not a significant difference in genotype and allele frequency of ATIR 1166A/C between the liver cirrhosisgranp and the control group （ P 〉 0.05 ）. Conclusion： Plasma concentration or angiotensin Ⅱ is related to the progression of liver cirrhosis and the 1166A/C polymorphism of the AT1R gene is not related to hereditary susceptibility or progression of liver cirrhosis.

关 键 词：肝炎 乙型 肝硬化 血管紧张素Ⅱ 受体 血管紧张素 

分 类 号：R575.2[医药卫生—消化系统]