不同碘浓度对培养血管内皮细胞增殖的影响  

Effects on endothelial cell proliferation induced by different concentration of iodine in vitro

作　　者：王晓磊[1] 徐丽雅 张海涛[1] 李加美[1] 郭成浩[1] 

机构地区：[1]山东大学医学院 [2]济南钢铁集团职工医院,山东济南250101

出　　处：《山东大学学报（医学版）》2007年第3期310-312,共3页Journal of Shandong University:Health Sciences

基　　金：山东省科技厅科研基金资助课题(2006BS03029)

摘　　要：目的:探讨不同碘浓度对人脐静脉血管内皮细胞增殖的影响。方法:体外培养人脐静脉血管内皮细胞株HUECV-304,在培养液中加入不同浓度的碘化钾,共分9组,分别为正常对照组及加碘组(0.5、1.0、2.0、3.0、4.0、5.0、6.0、8.0μmol/L),连续培养24h后,观察细胞形态改变并细胞计数,MTT比色法检测细胞活性。结果:0.5-4.0μmol/L剂量组在24 h内内皮细胞计数和MTT检测均明显高于对照组,且细胞形态没有异常。而5.0μmol/L以上组,虽在8 h内内皮细胞数目和MTT检测均明显高于对照组,但12 h后内皮细胞数目和MTT检测均明显低于对照组;显微镜观察在4 h始即可看见内皮细胞内有中毒颗粒出现,且随浓度增加和时间延长而增加,并出现明显的内皮形态变化。结论:碘对体外培养的内皮细胞有刺激增殖的作用,其作用与投碘的剂量及作用时间有关。Objective： To research the effects on proliferation of human umbilical vein endothelial cells （HUVECs） induced by different doses of iodine. Methods： HUVECs were subjected to iodine in various concentrations （0.5, 1.0, 2.0, 3.0, 4.0, 5.0, 6.0, and 8.0μmol/L）for 24 hours, and the morphology of the cultured endothelium was observed. MTT colofimetry was used to determine the proliferation activities of the cultured endothelium. Results： Iodine significantly increased the number and MTT activities of vascular endothelial cells from 0.5μmol/L to 4.0μmol/L compared with the normal group at 24 hours. Cell morphology was normal under a microscope. The number and MTT activities of vascular endothelial cells were significantly increased by iodine at over 5.0 μmol/L at 8 hours, but were decreased at 12 hours and later. Poison granules in the endothelium were produced by iodine at over 5.0μmol/L at 4 hours and increased in a dose- and time- manner. Conclusion： Iodine effects on the proliferation activity of endothelial cells are dose- and time-dependent.

关 键 词：碘 人脐静脉内皮细胞 增殖 细胞培养 

分 类 号：R363.16[医药卫生—病理学]