siRNA介导表皮生长因子受体基因沉默导致HepG2凋亡  

SiRNA mediated silencing of the epidermal growth factor receptor induces the apoptosis of HepG2

作　　者：牟壮博[1] 赵幼安[1] 王延军[2] 

机构地区：[1]山东大学齐鲁医院消化内科,山东济南250012 [2]山东省立医院中心实验室,山东济南250021

出　　处：《山东大学学报（医学版）》2007年第3期313-316,321,共5页Journal of Shandong University:Health Sciences

摘　　要：目的:研究含有与EGFR基因序列相同的19nt序列RNAi质粒针对HepG2细胞表皮生长因子受体的RNA干扰效果。方法:用pSIREN-hE转染细胞,其质粒骨架为RNAi-Ready pSIREN-Shuttle Vector,含有可以被U6启动子转录成shRNA的寡核苷酸链,大小为69 nt,含有2段19nt的寡核苷酸,用于编码EGFR基因的RNA干扰的siRNA的正义链及反义链,评价该RNAi质粒对人肝细胞癌细胞株(HepG2)EGFR基因的mRNA表达抑制效果及对细胞凋亡的作用。结果:HepG2细胞EGFR基因的mRNA被明显抑制并伴有凋亡增加。结论:含有与EGFR基因序列相同序列RNA质粒对EGFR基因的RNA干扰可能是治疗肝细胞癌的一种有效手段。Objective： To evaluate the RNAi efficiency of siRNA plasmid on the EGFR gene in a HepG2 cell line. Methods： By using a plasmid vector containing duplex of oligonucleotides, in which 19nt specific sequence of EG-FR expressing cassette to induce RNAi, the expression of endogenous EGFR in HepG2 human hepatocarcinoma cells was suppressed. The HepG2 cells were transfected with pSIREN-hE consisting of a RNAi-ready pSIREN-shuttle vector and duplex of oligonucleotides, which were built up of 69nt base pair, into a 19nt specific sequence of EGFR expressing cassette that can be transcripted by promoter U6. Results： The mRNA of EGFR in HepG2 human hepatocarcinoma cells was decreased and cell proliferation was reduced due to the induction of apoptosis. Conclusion： siRNA-mediated inhibition of EGFR gene may be a useful approach in the treatment of human hepatocarcinoma.

关 键 词：表皮生长因子受体 信使核糖核酸 RNA干扰 细胞凋亡 

分 类 号：R735.7[医药卫生—肿瘤]