Nucleostemin基因对人食管癌Eca-109细胞株转染条件优化的探索  

THE EXPERIMETAL STUDY ON NS GENE TRANSFCETION IN HUMAN ESOPHAGUS CANCER CELLS

作　　者：郑学芝[1] 胡静[2] 孙卫[1] 

机构地区：[1]牡丹江医学院生理教研室,157011 [2]牡丹江医学院组胚教研室

出　　处：《牡丹江医学院学报》2007年第2期3-5,共3页Journal of Mudanjiang Medical University

基　　金：牡丹江医学院科研项目

摘　　要：目的:探讨PCDNA4/C-NS-silencer质粒载体用于人食管癌Eca-109细胞株转染的最优化条件。方法:用RT-PCR方法检测基因的转染效率,分别研究不同DNA量/脂质体体积比值、不同质粒DNA-转染试剂复合物与靶细胞作用时间等情况对转染效果的影响。结果:当DNA量/脂质体体积比值在1:8,质粒DNA-转染试剂复合物与靶细胞作用时间为8h时细胞转染效率最高。结论:成功建立了NS基因转染人食管癌Eca-109细胞株转染的最优化条件。Objective：To detect the best methods of PCDNA4/C - NS - silencer transfection in human esophagus cancer cells. Methods：Different ritio of the DNA amount（μg） and the volume of Lipofectamine^TM2000 transfection Reagent （μg） , different action time of transfection reagent and DNA complex on target cells were analyzed by RT - PCR,which to detect the expression of NS gene mRNA. Results：When the DNA amount（μg） and the volume of Lipofectamine^TM2000 Reagent（μg） ritio was 1：8, ;When the action time of transfection reagent and DNA complex on target cells was 8h, the expression of NS gene mRNA was the highest. Conclusion：The best methods of PCDNA4/ C -NS -silencer transfection in human esophagus cancer cells was established.

关 键 词：基因转染 NUCLEOSTEMIN基因 食管癌细胞 阳离子脂质体 

分 类 号：R730.54[医药卫生—肿瘤]