三氧化二砷诱导乳腺癌细胞系MCF-7凋亡的实验研究  

Experimental Study on Apoptosis in Breast Cancer Cell Line MCF-7 Induced by Arsenic Trioxide

作　　者：曲志博[1] 刘连新[1] 陈炜[1] 郭化鑫[1] 杨海彦[1] 潘尚哈[1] 

机构地区：[1]哈尔滨医科大学第一临床医学院普通外科,哈尔滨150001

出　　处：《中国普外基础与临床杂志》2007年第2期159-162,共4页Chinese Journal of Bases and Clinics In General Surgery

基　　金：国家自然科学基金资助(编号:30300339)~~

摘　　要：目的观察不同浓度的三氧化二砷(As2O3)作用不同时间后对乳腺癌细胞系MCF-7的影响及其作用机理。方法应用不同浓度的As2O3作用于乳腺癌细胞后,观察As2O3对乳腺癌细胞生长状态的影响;用四甲基偶氮唑蓝(MTT)比色法观察其对MCF-7细胞增殖的影响;应用琼脂糖凝胶电泳和脱氧核糖核苷酸末端转移酶介导的缺口末端标记法(TUNEL)检测As2O3对MCF-7细胞凋亡的诱导作用。结果不同浓度的As2O3作用于乳腺癌细胞有明显的时间和剂量依赖性,As2O3作用后细胞生长明显受抑制,并出现明显的凋亡特征性改变(细胞膜完整、染色质固缩、核碎裂、凋亡小体形成);经1、2、4及8μmol/L4个剂量的As2O3处理48h后,琼脂糖凝胶电泳检测到细胞发生凋亡时DNA降解形成梯形分子条带;4μmol/LAs2O3作用24、48及72h后TUNEL法可检测到细胞凋亡水平显著性升高。结论As2O3可明显抑制乳腺癌细胞的生长,其机理主要是诱导乳腺癌细胞凋亡。Objective To observe the outcomes of using different concentrations of arsenic trioxide at varying phases on the breast cancer cell line MCF-7 and to study the mechanism of this effect. Methods The effect of arsenic trioxide on the growth of breast cancer cell line MCF-7 was observed after applying arsenic trioxide of different concentrations （0.5-16 μmol/L）. The inhibitory effect of arsenic trioxide on the cell proliferation was investigated with 3-（4,5-dimethyl-thizazol-2-yl）-2, 5-diphenyl tetrazolium bromide （MTT） and the induction of arsenic trioxide on cell apoptosis was detected by DNA ladder and terminal deoxynueleotidyl transferase mediated nick end labeling （TUNEL）. Results The effect of arsenic trioxide on breast cancer cell line MCF-7 depended on the phase and the dose. The number of cell decreased significantly and there were conspicuously typical morphological changes of apoptosis after the use of arsenic trioxide, including membrane blebbing, ehromatin pyknosis, nuclear fragmentation and the formation of apoptotie body. The typical DNA ladders were observed in the MCF-7 cells after 48 h administration of arsenic trioxide at concentrations 1-8 μmol/L. Significant elevations of apoptosis index at 24 h, 48 h and 72 h were all detected by TUNEL after incubating with 4 μmol/L arsenic trioxide. Conclusion Arsenic trioxide may inhibit the growth of breast cancer cell line MCF-7 significantly by inducing the apoptosis of breast cancer cell.

关 键 词：乳腺癌细胞 细胞凋亡 三氧化二砷 

分 类 号：R737.9[医药卫生—肿瘤]