生长激素受体在结直肠癌发生过程中表达上调  

UP-regulation of growth hormone receptor expression in colorectal tumors

作　　者：吴晓宇[1] 许哲[1] 陈彻[1] 祁晓平[1] 刘福坤[1] 

机构地区：[1]南京大学医学院临床学院(南京军区南京总医院)解放军普通外科研究所,江苏南京210002

出　　处：《肠外与肠内营养》2007年第2期73-76,82,共5页Parenteral & Enteral Nutrition

摘　　要：目的:研究生长激素受体(GHR)在结直肠正常黏膜-非肿瘤性息肉-腺瘤-结直肠癌序列中的表达,以探讨其在结直肠癌发生发展中的作用。方法:应用免疫组化(IHC)和逆转录聚合酶链反应(RT-PCR)检测正常结直肠黏膜、非肿瘤性息肉、结直肠腺瘤、结直肠癌共124例病人的标本中GHR表达。结果:免疫组化结果表明,与正常结直肠黏膜相比,结直肠腺瘤和结直肠癌的GHR蛋白表达显著上调(P<0.001);半定量RT-PCR结果同样表明,与正常结直肠黏膜相比,结直肠腺瘤和结直肠癌的GHR mRNA表达也显著上调(P<0.001)。结论:GH/GHR在结直肠癌的发生发展过程中可能起着重要的作用,对结直肠癌病人应谨慎使用重组人生长激素。Objective ： The aim of the present study was to analyze the expression of GHR in the colorectal adenoma-carcinoma sequence in order to determine whether its expression correlates with the various stages of cancer transformation. Methods：GHR distribution was assessed by immunohistochemistry and Reverse Transcriptase Polymerase Chain Reaction （RT-PCR） in normal, premalignant, and malignant colorectal lesions. Results：Most of the normal mucous tissues and hyperplastic polyps showed no or weak immunoreactivity for GHR. In contrast, most of the adenoma and adenocarcinoma samples reacted strongly or moderately showed an up-regulatio with monoclonal GHR antibodies. The semi-quantitative RT-PCR result also n of GHR mRNA expression during the polyp-adenoma-carcinoma sequence, which were in consistency with the IHC results（ P 〈 0.001 ）. Conclusion：GH/GHR plays an important role in the pathogenesis, transformation and development of colorectal carcinoma.

关 键 词：生长激素受体 结直肠癌 结直肠腺瘤 

分 类 号：R735.3[医药卫生—肿瘤]