尘螨连续石蜡切片的制备及染色技术  

Technique of preparation and staining of serial paraffin slices of dust mite

作　　者：张莺莺[1] 刘志刚[2] 孙新[1] 包莹[2] 李盟[2] 

机构地区：[1]蚌埠医学院病原生物学教研室安徽省感染与免疫重点实验室,安徽蚌埠233030 [2]深圳大学生命科学学院,广东深圳518060

出　　处：《昆虫知识》2007年第2期294-296,309,共4页Entomological Knowledge

基　　金：国家"863"计划项目(2002AA214011);国家自然科学基金项目(30471505;30271226);广东省科技重大专项(2003A3080502);深圳市科技计划资助项目(200218)

摘　　要：研究尘螨连续石蜡切片的制备技术。利用琼脂预包埋后再以塑化石蜡包埋,经切片和HE染色,获得结构完整、定位准确、染色清晰的连续切片。探讨制片过程中一些步骤的改进和注意事项,为尘螨显微结构的形态学研究提供可能,也为免疫组化、原位PCR及过敏性疾病尘螨特异性变应原的定位等研究奠定良好的基础。Dust mite was embeded beforehand in agar and then in paraplast. Serial paraffin sections were made and HE staining was processed. The tissues exhibited integrity, exact orientation and different staining. We discussed the amelioration in the process. This technique is valuable in studying the morphology, immunohistochemistry, in situ PCR and localization of the allergen.

关 键 词：尘螨 琼脂 塑化石蜡 HE染色 

分 类 号：Q96-3[生物学—昆虫学]