siRNA沉默人乳腺癌细胞株CXCR4基因的实验研究  

Silencing breast cancer cell CXCR4 gene in vitro

作　　者：厉红元[1] 任国胜[1] 谭金详[1] 

机构地区：[1]重庆医科大学附属第一医院普通外科,重庆400016

出　　处：《第三军医大学学报》2007年第8期728-730,共3页Journal of Third Military Medical University

基　　金：重庆市卫生局科研项目(07-2-029)~~

摘　　要：目的探讨shRNA-CXCR4对人乳腺癌细胞MCF-7、MDA-MB-231、MDA-MB-435 s细胞CXCR4基因表达及蛋白表达的影响。方法通过shRNA-CXCR4沉默CXCR4基因后,RT-PCR、W estern b lotting检测3株人乳腺癌细胞MCF-7、MDA-MB-231、MDA-MB-435 s的CXCR4 mRNA及其蛋白的表达。结果shRNA-CXCR4作用于人乳腺癌细胞株MCF-7、MDA-MB-231、MDA-MB-435 s后能明显抑制其CXCR4基因的mRNA及蛋白表达水平(P<0.05)。结论shRNA-CXCR4能特异性抑制乳腺癌细胞的CXCR4 mRNA及其蛋白的表达。Objective To study whether shRNA-CXCR4 could inhibit the CXCR4 gene expression in MCF-7, MDA-MB-231 and MDA-MB-435s breast cancer cells. Methods After the knockdown of CXCR4 by shRNA, the CXCR4 mRNA expression by RT-PCR and the CXCR4 protein expression by Western blotting in breast cancer cells were examined. Results The CXCR4 mRNA expression and its protein expression were decreased significantly in MCF-7, MDA-MB-231 and MDA-MB-435s breast cancer cells by shRNA-CXCR4（P 〈 0.05）. Conclusion Silencing CXCR4 could inhibit the breast cancer cell CXCR4 mRNA and its protein expression.

关 键 词：乳腺癌 SHRNA CXCR4基因 

分 类 号：R394-33[医药卫生—医学遗传学] R73-354[医药卫生—基础医学]